Calpastatin (CAST) (NM_001042443) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BS-17; PLACK
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001042443, the custom clone sequence may differ by one or more nucleotides ATGAATCCCACAGAAACCAAGGCCATTCCAGTCAGCCAACAGATGGAAGGACCACATCTT CCTAACAAGAAAAAACACAAAAAACAGGCTGTAAAAACAGAACCTGAGAAGAAGTCACAG TCAACCAAGCTGTCTGTGGTTCATGAGAAAAAATCCCAAGAAGGAAAGCCAAAAGAACAC ACAGAGCCAAAAAGCCTACCCAAGCAGGCATCAGATACAGGAAGTAACGATGCTCACAAT AAAAAAGCAGTTTCCAGATCAGCTGAACAGCAGCCATCAGAGAAATCAACAGAACCAAAG ACTAAACCACAAGACATGATTTCTGCTGGTGGAGAGAGTGTTGCTGGTATCACTGCAATA TCTGGCAAGCCGGGTGACAAGAAAAAAGAAAAGAAATCATTAACCCCAGCTGTGCCAGTT GAATCTAAACCGGATAAACCATCGGGAAAGTCAGGCATGGATGCTGCTTTGGATGACTTA ATAGATACTTTAGGAGGACCTGAAGAAACTGAAGAAGAAAATACAACGTATACTGGACCA GAAGTTTCAGATCCAATGAGTTCCACCTACATAGAGGAATTGGGTAAAAGAGAAGTCACA ATTCCTCCAAAATATAGGGAACTATTGGCTAAAAAGGAAGGGATCACAGGGCCTCCTGCA GACTCTTCGAAACCCATAGGGCCAGATGATGCTATAGACGCCTTGTCATCTGACTTCACC TGTGGGTCGCCTACAGCTGCTGGAAAGAAAACTGAAAAAGAGGAATCTACAGAAGTTTTA AAAGCTCAGTCAGCAGGGACAGTCAGAAGTGCTGCTCCACCCCAAGAGAAGAAAAGAAAG GTGGAGAAGGATACAATGAGTGATCAAGCACTCGAGGCTCTGTCGGCTTCACTGGGCACC CGGCAAGCAGAACCTGAGCTCGACCTCCGCTCAATTAAGGAAGTCGATGAGGCAAAAGCT AAAGAAGAAAAACTAGAGAAGTGTGGTGAGGATGATGAAACAATCCCATCTGAGTACAGA TTAAAACCAGCCACGGATAAAGATGGAAAACCACTATTGCCAGAGCCTGAAGAAAAACCC AAGCCTCGGAGTGAATCAGAACTCATTGATGAACTTTCAGAAGATTTTGACCGGTCTGAA TGTAAAGAGAAACCATCTAAGCCAACTGAAAAGACAGAAGAATCTAAGGCCGCTGCTCCA GCTCCTGTGTCGGAGGCTGTGTGTCGGACCTCCATGTGTAGTATACAGTCAGCACCCCCT GAGCCGGCTACCTTGAAGGGCACAGTGCCAGATGATGCTGTAGAAGCCTTGGCTGATAGC CTGGGGAAAAAGGAAGCAGATCCAGAAGATGGAAAACCTGTGATGGATAAAGTCAAGGAG AAGGCCAAAGAAGAAGACCGTGAAAAGCTTGGTGAAAAAGAAGAAACAATTCCTCCTGAT TATAGATTAGAAGAGGTCAAGGATAAAGATGGAAAGCCACTCCTGCCAAAAGAGTCTAAG GAACAGCTTCCACCCATGAGTGAAGACTTCCTTCTGGATGCTTTGTCTGAGGACTTCTCT GGTCCACAAAATGCTTCATCTCTTAAATTTGAAGATGCTAAACTTGCTGCTGCCATCTCT GAAGTGGTTTCCCAAACCCCAGCTTCAACGACCCAAGCTGGAGCCCCACCCCGTGATACC TCGCAGAGTGACAAAGACCTCGATGATGCCTTGGATAAACTCTCTGACAGTCTAGGACAA AGGCAGCCTGACCCAGATGAGAACAAACCAATGGAAGATAAAGTAAAGGAAAAAGCTAAA GCTGAACATAGAGACAAGCTTGGAGAAAGAGATGACACTATCCCACCTGAATACAGACAT CTCCTGGATGATAATGGACAGGACAAACCAGTGAAGCCACCTACAAAGAAATCAGAGGAT TCAAAGAAACCTGCAGATGACCAAGACCCCATTGATGCTCTCTCAGGAGATCTGGACAGC TGTCCCTCCACTACAGAAACCTCACAGAACACAGCAAAGGATAAGTGCAAGAAGGCTGCT TCCAGCTCCAAAGCACCTAAGAATGGAGGTAAAGCGAAGGATTCAGCAAAGACAACAGAG GAAACTTCCAAGCCAAAAGATGACTAA
Restriction Sites	Please inquire
ACCN	NM_001042443
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001042443.1, NP_001035908.1
RefSeq Size	4357 bp
RefSeq ORF	2127 bp
Locus ID	831
UniProt ID	P20810
Gene Summary	The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]

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