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OSBPL9 (NM_148909) Human Untagged Clone
CAT＃: SC314600
OSBPL9 (untagged)-Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7
CNY 14,250.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ORP-9; ORP9 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314600 representing NM_148909.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGTCCATCATGGAAGGGCCGCTGAGCAAATGGACTAACGTGATGAAGGGCTGGCAGTACCGTTGG TTCGTGCTGGACTACAATGCAGGACTGCTCTCCTACTACACGTCCAAGGACAAAATGATGAGAGGCTCT CGCAGAGGATGTGTTAGACTCAGAGGAGCTGTGATTGGTATAGACGATGAGGACGACAGCACCTTCACA ATAACTGTTGATCAGAAAACCTTCCATTTCCAGGCCCGTGATGCTGATGAGCGAGAGAAGTGGATCCAT GCCTTAGAAGAAACAATTCTTCGACATACTCTCCAGCTTCAAATCAGTACCACACTTGCTTTTTTCCAG TCTTCTGGTATCTCTCCAGTTCTTGAATTTTCAAAAATAATTGGTTTGGATTCAGGATTTGTTCCTAGT GTCCAAGATTTTGATAAGAAACTTACAGAAGCTGATGCTTACCTACAAATCTTGATTGAACAATTAAAG CTTTTTGATGACAAGCTTCAAAACTGCAAAGAAGATGAACAGAGAAAGAAAATTGAAACTCTCAAAGAG ACAACAAATAGCATGGTAGAATCAATTAAACACTGCATTGTGTTGCTGCAGATTGCCAAAAGTACTATT AATCCCGTAGATGCAATATATCAACCTAGTCCTTTGGAACCTGTGATCAGCACAATGCCTTCCCAGACT GTGTTACCTCCAGAACCTGTTCAGTTGTGTAAGTCAGAGCAGCGTCCATCTTCCCTACCAGTTGGACCT GTGTTGGCTACCTTGGGACATCATCAGACTCCTACACCAAATAGTACAGGCAGTGGCCATTCACCACCG AGTAGCAGTCTCACTTCTCCAAGCCACGTGAACTTGTCTCCAAATACAGTCCCAGAGTTCTCTTACTCC AGCAGTGAAGATGAATTTTATGATGCTGATGAATTCCATCAAAGTGGCTCATCCCCAAAGCGCTTAATA GATTCTTCTGGATCTGCCTCAGTCCTGACACACAGCAGCTCGGGAAATAGTCTAAAACGCCCAGATACC ACAGAATCACTTAATTCTTCCTTGTCCAATGGAACAAGTGATGCTGACCTGTTTGATTCACATGATGAC AGAGATGATGATGCGGAGGCAGGGTCTGTGGAGGAGCACAAGAGCGTTATCATGCATCTCTTGTCGCAG GTTAGACTTGGAATGGATCTTACTAAGGTAGTTCTTCCAACGTTTATTCTTGAAAGAAGATCTCTTTTA GAAATGTATGCAGACTTTTTTGCACATCCGGACCTGTTTGTGAGCATTAGTGACCAGAAGGATCCCAAG GATCGAATGGTTCAGGTTGTGAAATGGTACCTCTCAGCCTTTCATGCGGGAAGGAAAGGATCAGTTGCC AAAAAGCCATACAATCCCATTTTGGGCGAGATTTTTCAGTGTCATTGGACATTACCAAATGATACTGAA GAGAACACAGAACTAGTTTCAGAAGGACCAGTTCCCTGGGTTTCCAAAAACAGTGTAACATTTGTGGCT GAGCAGGTTTCCCATCATCCACCCATTTCAGCCTTTTATGCTGAGTGTTTTAACAAGAAGATACAATTC AATGCTCATATCTGGACCAAATCAAAATTCCTTGGGATGTCAATTGGGGTGCACAACATAGGGCAGGGC TGTGTCTCATGTCTAGACTATGATGAACATTACATTCTCACATTCCCCAATGGCTATGGAAGGTCTATC CTCACAGTGCCCTGGGTGGAATTAGGAGGAGAATGCAATATTAATTGTTCCAAAACAGGCTATAGTGCA AATATCATCTTCCACACTAAACCCTTCTATGGGGGCAAGAAGCACAGAATTACTGCCGAGATTTTTTCT CCAAATGACAAGAAGTCTTTTTGCTCAATTGAAGGGGAATGGAATGGTGTGATGTATGCAAAATATGCA ACAGGGGAAAATACAGTCTTTGTAGATACCAAGAAGTTGCCTATAATCAAGAAGAAAGTGAGGAAGTTG GAAGATCAGAACGAGTATGAATCCCGCAGCCTTTGGAAGGATGTCACTTTCAACTTAAAAATCAGAGAC ATTGATGCAGCAACTGAAGCAAAGCACAGGCTTGAAGAAAGACAAAGAGCAGAAGCCCGAGAAAGGAAG GAGAAGGAAATTCAGTGGGAGACAAGGTTATTTCATGAAGATGGAGAATGCTGGGTTTATGATGAACCA TTACTGAAACGTCTTGGTGCTGCCAAGCATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_148909 |
| Insert Size | 2241 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_148909.3 |
| RefSeq Size | 3925 bp |
| RefSeq ORF | 2241 bp |
| Locus ID | 114883 |
| UniProt ID | Q96SU4 |
| MW | 84.3 kDa |
| Gene Summary | This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. This family member functions as a cholesterol transfer protein that regulates Golgi structure and function. Multiple transcript variants, most of which encode distinct isoforms, have been identified. Related pseudogenes have been identified on chromosomes 3, 11 and 12. [provided by RefSeq, Jul 2010] Transcript Variant: This variant (7) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (f) has a longer and distinct N-terminus, compared to isoform a. Isoform f contains both a pleckstrin homology domain and a sterol-binding domain. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. This transcript contains a uAUG that would result in an 18 aa N-terminal extension, but it has a weak Kozak signal, is not supported by the vast majority of human and homologous transcripts, and dAUG use is supported by data in PMID:19413330, which selectively purifies acetylated protein N-termini and shows that Ala-2 is acetylated following cleavage of Met-1 as encoded by the dAUG. CCDS Note: This CCDS representation uses a downstream start codon but includes a potential upstream start codon that would result in a protein that is 18 aa longer at the N-terminus. However, the upstream start codon has a weak Kozak signal, the vast majority of transcripts from human and homologous species start downstream of it, and data in PMID:19413330 indicate that the N-terminus begins at an N-alpha acetylated Ala-2 residue (nASIMEGPLSK...) following cleavage of the initiating Met-1 derived from the second start codon. The second start codon is therefore represented in this CCDS. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211520 | OSBPL9 (Myc-DDK-tagged)-Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7 |
CNY 5,464.00 |
|
| RC211520L1 | Lenti ORF clone of Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7, Myc-DDK-tagged |
CNY 7,864.00 |
|
| RC211520L2 | Lenti ORF clone of Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7, mGFP tagged |
CNY 9,690.00 |
|
| RC211520L3 | Lenti ORF clone of Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7, Myc-DDK-tagged |
CNY 9,690.00 |
|
| RC211520L4 | Lenti ORF clone of Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7, mGFP tagged |
CNY 9,690.00 |
|
| RG211520 | OSBPL9 (tGFP-tagged) - Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7 |
CNY 8,550.00 |
