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MATK (NM_139355) Human Untagged Clone
CAT＃: SC323486
MATK (untagged)-Kinase deficient mutant (K263M) of Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 1
CNY 3,784.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CHK; CTK; HHYLTK; HYL; HYLTK; Lsk |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323486 sequence for NM_139355 edited (data generated by NextGen Sequencing)
ATGGCGGGGCGAGGCTCTCTGGTTTCCTGGCGGGCATTTCACGGCTGTGATTCTGCTGAG GAACTTCCCCGGGTGAGCCCCCGCTTCCTCCGAGCCTGGCACCCCCCTCCCGTCTCAGCC AGGATGCCAACGAGGCGCTGGGCCCCGGGCACCCAGTGTATCACCAAATGCGAGCACACC CGCCCCAAGCCAGGGGAGCTGGCCTTCCGCAAGGGCGACGTGGTCACCATCCTGGAGGCC TGCGAGAACAAGAGCTGGTACCGCGTCAAGCACCACACCAGTGGACAGGAGGGGCTGCTG GCAGCTGGGGCGCTGCGGGAGCGGGAGGCCCTCTCCGCAGACCCCAAGCTCAGCCTCATG CCGTGGTTCCACGGGAAGATCTCGGGCCAGGAGGCTGTCCAGCAGCTGCAGCCTCCCGAG GATGGGCTGTTCCTGGTGCGGGAGTCCGCGCGCCACCCCGGCGACTACGTCCTGTGCGTG AGCTTTGGCCGCGACGTCATCCACTACCGCGTGCTGCACCGCGACGGCCACCTCACAATC GATGAGGCCGTGTTCTTCTGCAACCTCATGGACATGGTGGAGCATTACAGCAAGGACAAG GGCGCTATCTGCACCAAGCTGGTGAGACCAAAGCGGAAACACGGGACCAAGTCGGCCGAG GAGGAGCTGGCCAGGGCGGGCTGGTTACTGAACCTGCAGCATTTGACATTGGGAGCACAG ATCGGAGAGGGAGAGTTTGGAGCTGTCCTGCAGGGTGAGTACCTGGGGCAAAAGGTGGCC GTGAAGAATATCAAGTGTGATGTGACAGCCCAGGCCTTCCTGGACGAGACGGCCGTCATG ACGAAGATGCAACACGAGAACCTGGTGCGTCTCCTGGGCGTGATCCTGCACCAGGGGCTG TACATTGTCATGGAGCACGTGAGCAAGGGCAACCTGGTGAACTTTCTGCGGACCCGGGGT CGAGCCCTCGTGAACACCGCTCAGCTCCTGCAGTTTTCTCTGCACGTGGCCGAGGGCATG GAGTACCTGGAGAGCAAGAAGCTTGTGCACCGCGACCTGGCCGCCCGCAACATCCTGGTC TCAGAGGACCTGGTGGCCAAGGTCAGCGACTTTGGCCTGGCCAAAGCCGAGCGGAAGGGG CTAGACTCAAGCCGGCTGCCCGTCAAGTGGACGGCGCCCGAGGCTCTCAAACACGGGAAG TTCACCAGCAAGTCGGATGTCTGGAGTTTTGGGGTGCTGCTCTGGGAGGTCTTCTCATAT GGACGGGCTCCGTACCCTAAAATGTCACTGAAAGAGGTGTCGGAGGCCGTGGAGAAGGGG TACCGCATGGAACCCCCCGAGGGCTGTCCAGGCCCCGTGCACGTCCTCATGAGCAGCTGC TGGGAGGCAGAGCCCGCCCGCCGGCCACCCTTCCGCAAACTGGCCGAGAAGCTGGCCCGG GAGCTACGCAGTGCAGGTGCCCCAGCCTCCGTCTCAGGGCAGGACGCCGACGGCTCCACC TCGCCCCGAAGCCAGGAGCCCTGA Clone variation with respect to NM_139355.2 >OriGene 5' read for mutant NM_139355 unedited
ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCCGGCGGCGGCG GCGGCGCGCGGAGGAGACCGCAGTGCGGCCGGCGCTAGGACCCGCGGGGGCCTCCCAGGCCGCGGCGCCT CGCGCTTTCCCCCACTCCCCGACCCTTCTTCGCCCCCAAAATGAGGAAACGGAGCAACTCGCTCCAAGTT GTGCAGCCGGGACCGCCTCGGGGTGTGCAGCCGGGCTCGCGGAGGCCCTCCCTGGGGGCGGGCGCCGGGG CGCGGCTCGGGGGCCGCCCCCCTGAGCCAAAAACCAGGAAAAACCAGCCTCCGGTCCAGTGGGCACCCCA CCTCCCCTACCCTCCGGTGCAACCCGCCTGGCCGGTGGCAGGCCTTTCCCACCGTCCCCAACGGTAACCA CTTGTCAAGTTGGCCTTTCCCCGCCTCATTTTCCTTCGGGGCCCAATGCCGGGCCCAACCTCTGGGTTTC CGGGGGGCATTACCGGTGGGAATTTCGCGGGAAACTTCCCGGGGAGCCCCGCTTTCTCCAAACCGGACCC CCCCCCCGTTAAACGATACCACAAAGCCGGGGCCCGGCCCCCCAGTGTACCCATACGCACCCCTCCCAAA CGAGAGAGGCGCCTCAAGGGGCAGTGCAACCTTAGAGCGGAGAGACGAGCGAGCGACTGCCCACCCCC >SC323486 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CAMGGGCTGGTMTGACTGCAGCATTTGACATTGGGAGCACAGATCGGAGAGGGAGAGTTTGGAGCTGTCC TGCAGGGTGAGTACCTGGGGCAAAAGGTGGCCGTGATGAATATCAAGTGTGATGTGACAGCCCAGGCCTT CCTGGACGAGACGGCCGTCATGACGAAGATGCAACACGAGAACCTGGTGCGTCTCCTGGGCGTGATCCTG CACCAGGGGCTGTACATTGTCATGGAGCACGTGAGCAAGGGCAAC |
| Restriction Sites | Please inquire |
| ACCN | NM_139355 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_139355.1, NP_647612.1 |
| RefSeq Size | 2183 bp |
| RefSeq ORF | 1524 bp |
| Locus ID | 4145 |
| UniProt ID | P42679 |
| Protein Families | Druggable Genome, Protein Kinase, Stem cell - Pluripotency |
| Gene Summary | The protein encoded by this gene has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site. This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the predominant isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223161 | MATK (Myc-DDK-tagged)-Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 1 |
CNY 3,776.00 |
|
| RC223161L1 | Lenti ORF clone of Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223161L2 | Lenti ORF clone of Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC223161L3 | Lenti ORF clone of Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223161L4 | Lenti ORF clone of Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG223161 | MATK (tGFP-tagged) - Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 1 |
CNY 4,370.00 |
|
| SC100071 | MATK (untagged)-Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 1 |
CNY 3,784.00 |
