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Phospholipase C epsilon 1 (PLCE1) (NM_001165979) Human Untagged Clone
CAT＃: SC327342
PLCE1 (untagged)-Human phospholipase C epsilon 1 (PLCE1) transcript variant 2
CNY 38,480.00
货期*
8周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NPHS3; PLCE; PPLC |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001165979, the custom clone sequence may differ by one or more nucleotides
ATGGTTTCAGAAGGAAGTGCAGCAGGAAGAGACTTTGCAGGAATGGAAGAGGTGAGGCAG CTCCACGTGAGATTCTGCAAAGGGATTAAGATTTGGCACCAGGCTTGGTTTCTGTGCAGC CTCTTAGGCAGAGAGCCCCAGGAGAGGGAAGCAGGCTGTCAGCTGTGGCTTTGCACGCTC TCAGCTGTCTTGAAGGTTGGTTGGCTCTTCCCGCTCTCTGAGGTACCCAATTTTACCTTG TTAAAGGATGGATGTGGATGCTGGAGGCTTAAAGAAGACCAGATCTACAATGCAGTGAGA AGAGAAGAAACAGAAAATACAGTTGGATCTCTACTCCATTTCCTCACCAAGCTCCCAGCC TCCGAGACAGCCCATGGAAGGATAAGCGTTGGTCCATGCTTAAAGCAATGTGTCCGAGAC ACTGTATGTGAGTATCGCGCCACCCTCCAAAGGACTTCAATATCGCAGTACATCACCGGT TCTCTCCTAGAAGCAACCACGTCTTTGGGAGCAAGAAGTGGCCTTCTCAGTACTTTTGGA GGATCCACTGGACGAATGATGCTGAAAGAACGCCAGCCAGGCCCCTCTGTGGCCAATTCC AATGCCCTCCCTTCAAGTTCAGCTGGGATCAGCAAGGAGCTGATCGATCTGCAGCCTCTC ATCCAGTTCCCAGAGGAAGTCGCCAGCATCCTGATGGAGCAAGAGCAGACTATTTACCGC AGGGTCTTGCCAGTCGACTACCTTTGCTTCTTAACACGGGACTTGGGCACTCCTGAATGC CAGAGCTCCTTGCCCTGCCTCAAAGCATCCATCTCAGCGTCGATTCTTACCACTCAGAAT GGAGAGCACAATGCCCTTGAAGATCTGGTGATGAGGTTTAATGAGGTGAGCTCCTGGGTG ACATGGCTGATCCTCACGGCAGGCTCCATGGAGGAGAAGCGAGAAGTCTTTTCATATTTG GTGCATGTGGCCAAATGCTGCTGGAACATGGGCAACTACAACGCTGTCATGGAGTTCTTG GCTGGCCTCAGGTCAAGAAAAGTTTTAAAAATGTGGCAGTTCATGGACCAGTCTGATATT GAGACCATGAGGAGCCTGAAGGATGCTATGGCCCAGCATGAGTCCTCTTGTGAGTACAGA AAGGTGGTGACACGTGCCCTGCACATCCCTGGCTGTAAGGTGGTTCCATTCTGTGGGGTG TTTCTGAAGGAGCTCTGTGAAGTGCTTGACGGCGCCTCCGGTCTCATGAAGCTTTGCCCG CGGTACAATTCCCAAGAAGAAACTTTAGAGTTTGTAGCAGATTACAGTGGACAAGATAAT TTCTTACAACGAGTGGGACAAAATGGCTTAAAGAATTCGGAGAAGGAGTCCACTGTCAAC AGCATCTTTCAGGTCATCCGGAGCTGCAATCGAAGTCTGGAGACAGACGAGGAGGACAGC CCCAGTGAAGGAAACAGCTCCAGGAAAAGCTCCTTGAAGGATAAAAGCCGATGGCAGTTT ATAATTGGAGATTTGTTGGATTCAGACAATGACATCTTTGAGCAATCCAAAGAATACGAC TCTCATGGTTCAGAGGACTCACAGAAGGCCTTCGACCATGGGACGGAGCTCATCCCTTGG TACGTGCTGTCCATCCAAGCCGATGTGCACCAGTTCCTGCTGCAGGGGGCCACGGTCATC CACTACGACCAGGACACACACCTCTCTGCCCGCTGCTTCCTCCAGCTTCAGCCCGACAAT AGCACCTTGACCTGGGTAAAGCCCACAACTGCCTCCCCAGCCAGCAGTAAAGCAAAACTT GGTGTACTTAATAACACAGCTGAGCCTGGAAAATTCCCACTACTGGGTAATGCTGGATTA AGTAGCCTGACGGAAGGGGTCTTGGATCTTTTTGCAGTGAAGGCTGTATACATGGGCCAC CCTGGCATTGATATACACACTGTGTGTGTTCAGAACAAACTGGGTAGCATGTTCCTGTCA GAGACTGGTGTGACATTGCTCTATGGGCTTCAGACCACAGACAACAGATTATTGCACTTC GTGGCACCAAAGCACACAGCTAAAATGCTCTTCAGCGGATTATTGGAACTCACTAGAGCT GTGAGAAAGATGAGGAAATTCCCTGACCAAAGACAGCAGTGGCTGCGGAAACAGTACGTC AGCCTTTATCAGGAGGATGGACGGTATGAAGGCCCAACTTTGGCTCACGCTGTGGAGTTG TTTGGTGGCAGACGGTGGAGTGCTCGAAACCCCAGCCCCGGAACATCAGCAAAGAATGCT GAGAAGCCCAATATGCAGAGAAACAATACCCTGGGCATAAGCACTACCAAGAAAAAGAAG AAAATCCTCATGAGGGGTGAGAGTGGAGAGGTAACTGACGATGAGATGGCAACCCGAAAG GCCAAGATGCACAAAGAGTGTCGAAGCCGGAGTGGTTCTGATCCTCAAGACATTAATGAA CAAGAAGAATCAGAGGTGAATGCCATCGCTAACCCTCCAAACCCCCTCCCTTCCAGAAGA GCCCACTCTTTGACCACAGCTGGGTCCCCCAACTTGGCTGCCGGGACGTCATCTCCCATC AGGCCAGTGTCCTCCCCTGTGCTGTCTTCTTCAAACAAGAGCCCATCCAGTGCTTGGAGC AGTAGTAGCTGGCACGGGCGGATCAAAGGCGGCATGAAGGGATTTCAGAGCTTCATGGTT TCAGATAGCAACATGAGTTTTGTTGAATTTGTTGAGCTGTTCAAATCATTCAGTGTCAGG AGCCGCAAGGACCTGAAGGATCTGTTTGATGTCTATGCAGTGCCCTGCAACCGATCTGGC TCCGAGTCAGCCCCACTCTACACCAACCTGACAATTGATGAAAACACCAGCGATCTTCAG CCTGACCTAGATCTGTTGACCAGAAATGTCTCGGATTTGGGGTTGTTCATTAAGAGTAAA CAGCAGCTATCGGACAACCAGAGGCAGATATCTGATGCCATTGCTGCTGCAAGCATTGTG ACAAATGGCACTGGGATTGAGAGCACATCTCTGGGCATTTTTGGGGTGGGCATACTTCAG CTCAACGATTTCCTCGTGAATTGCCAAGGAGAACACTGCACTTATGATGAAATCCTCAGC ATCATCCAGAAGTTCGAGCCTAGCATCAGTATGTGTCATCAGGGACTAATGTCATTTGAA GGGTTTGCCAGGTTTCTGATGGATAAAGAAAATTTTGCCTCAAAAAATGATGAGTCACAG GAGAACATTAAAGAACTGCAGCTACCCCTCTCATACTATTACATCGAATCTTCGCACAAT ACCTACCTCACGGGCCATCAGCTCAAAGGAGAATCCTCGGTAGAACTCTACAGCCAGGTC CTTTTGCAAGGCTGTCGAAGTGTAGAATTGGACTGCTGGGACGGAGACGATGGGATGCCC ATCATTTATCATGGACATACGCTGACAACCAAGATCCCCTTCAAGGAAGTGGTTGAAGCC ATTGATCGCAGTGCCTTCATCAACTCTGACCTGCCAATCATCATATCGATTGAGAACCAC TGTTCATTGCCTCAGCAACGAAAAATGGCAGAAATTTTCAAGACTGTGTTTGGAGAAAAG CTGGTGACTAAATTCTTATTTGAGACTGATTTCTCAGATGATCCAATGCTTCCTTCACCT GACCAACTCAGAAAGAAAGTTCTTCTTAAAAACAAGAAGCTAAAAGCCCATCAGACGCCA GTGGATATCTTAAAGCAAAAGGCTCATCAGTTAGCATCTATGCAAGTGCAGGCTTATAAT GGTGGGAATGCCAACCCCCGACCTGCCAATAATGAGGAAGAGGAAGATGAGGAGGACGAA TATGATTATGACTATGAATCCCTTTCTGATGACAACATTCTGGAAGACAGACCTGAAAAT AAATCATGTAATGACAAGCTTCAGTTTGAATATAATGAAGAAATCCCAAAGAGGATAAAG AAAGCAGATAACTCTGCTTGCAACAAAGGAAAGGTTTATGATATGGAACTGGGAGAAGAA TTTTATCTTGATCAGAATAAAAAGGAAAGCAGACAGATTGCACCAGAGCTTTCTGACCTT GTAATCTATTGTCAAGCAGTAAAATTTCCAGGACTGTCAACTCTAAATGCATCTGGCTCT AGCAGAGGAAAAGAAAGGAAAAGCAGGAAGTCCATTTTTGGCAACAATCCGGGCAGAATG AGCCCAGGGGAGACAGCATCATTTAACAAAACATCTGGAAAAAGTTCCTGTGAAGGCATT CGACAGACCTGGGAGGAATCTTCTTCCCCTCTCAACCCAACCACGTCCCTCAGTGCTATC ATTAGAACTCCCAAATGTTATCATATCTCGTCGCTGAATGAAAATGCCGCCAAACGTCTG TGTCGCAGGTATTCTCAGAAACTGACCCAGCACACCGCCTGTCAGCTGCTGAGAACTTAC CCTGCTGCCACCCGCATCGACTCTTCCAACCCGAACCCCCTCATGTTCTGGCTCCATGGG ATACAGCTTGTGGCACTCAACTACCAGACTGATGATCTCCCTTTACATTTAAATGCTGCA ATGTTTGAGGCAAATGGTGGTTGTGGTTATGTATTGAAACCTCCAGTTCTGTGGGACAAG AACTGCCCCATGTATCAGAAGTTTTCTCCACTAGAAAGAGATCTGGACAGCATGGATCCT GCAGTCTATTCTTTAACTATTGTCTCTGGTCAGAATGTGTGCCCCAGTAATAGCATGGGA AGCCCGTGCATTGAAGTCGACGTCCTGGGCATGCCTCTGGACAGCTGCCATTTCCGCACA AAGCCCATCCATCGAAACACCCTGAACCCCATGTGGAACGAGCAGTTTCTGTTCCACGTT CACTTCGAAGATCTTGTATTTCTTCGTTTTGCAGTTGTGGAAAACAATAGTTCAGCGGTA ACTGCTCAGAGAATCATTCCACTGAAAGCTTTAAAACGAGGATATCGACATCTTCAGCTG CGAAACCTTCACAATGAAGTCTTGGAGATTTCTAGTTTATTCATTAACAGCAGAAGGATG GAAGAAAATTCCTCTGGCAATACCATGTCAGCCTCTTCGATGTTTAATACAGAAGAAAGA AAATGTTTGCAGACTCACAGAGTCACGGTGCATGGGGTCCCAGGGCCAGAGCCCTTTACC GTTTTCACTATTAATGGAGGCACCAAGGCAAAGCAGCTTCTGCAGCAAATTCTGACAAAT GAACAAGACATCAAACCTGTTACCACAGACTATTTTTTGATGGAAGAAAAATATTTTATA TCTAAAGAAAAGAATGAATGTAGGAAACAACCATTCCAGAGAGCCATTGGTCCAGAAGAG GAGATCATGCAAATTTTAAGCAGCTGGTTTCCAGAAGAGGGATACATGGGCAGGATTGTC TTAAAAACCCAGCAGGAAAACCTAGAAGAGAAAAACATTGTTCAAGATGACAAAGAGGTG ATCTTGAGCTCAGAGGAGGAGAGTTTCTTTGTCCAAGTGCATGATGTTTCTCCAGAGCAA CCTCGAACAGTCATCAAAGCACCCCGCGTCAGCACTGCACAGGATGTCATTCAGCAGACC TTATGCAAAGCCAAATATTCCTACAGCATCCTGAGCAACCCCAATCCAAGCGACTATGTG CTTTTGGAAGAGGTGGTGAAAGACACTACCAACAAGAAGACTACCACACCAAAGTCCTCT CAGCGGGTCCTTCTGGATCAGGAGTGTGTGTTTCAAGCCCAAAGCAAGTGGAAAGGTGCA GGAAAATTCATCCTTAAGCTAAAGGAGCAGGTGCAGGCATCTCGAGAAGATAAAAAGAAA GGCATTTCTTTCGCAAGTGAACTCAAGAAGCTCACCAAGTCAACTAAACAGCCCCGAGGA CTTACATCACCTTCTCAGCTCTTGACCTCAGAAAGTATCCAAACCAAGGAGGAGAAACCT GTGGGTGGCTTGTCCTCCAGTGACACAATGGATTACCGACAG |
| Restriction Sites | Please inquire |
| ACCN | NM_001165979 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001165979.1, NP_001159451.1 |
| RefSeq Size | 6456 bp |
| RefSeq ORF | 5985 bp |
| Locus ID | 51196 |
| UniProt ID | Q9P212 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| Gene Summary | This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009] Transcript Variant: This variant (2) lacks two exons and has an additional novel exon at its 5' end, compared to variant 1. These differences produce a unique 5' UTR and cause translation initiation at a novel start codon, compared to variant 1. The encoded protein (isoform 2; also known as PLCepsilon1b) has a shorter and distinct N-terminus, compared to isoform 1. |
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