CNOT2 (NM_001199302) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDC36; HSPC131; IDNADFS; NOT2; NOT2H
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329509 representing NM_001199302. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGAGGACTGATGGACATACATTATCTGAGAAAAGAAACTACCAGGTGACAAACAGCATGTTTGGT GCTTCAAGAAAGAAGTTTGTAGAGGGGGTCGACAGTGACTACCATGACGAAAACATGTACTACAGCCAG TCTTCTATGTTTCCACATCGGTCAGAAAAAGATATGCTGGCATCACCATCTACATCAGGTCAGCTGTCT CAGTTTGGGGCAAGTTTATACGGGCAACAAAGTGCACTAGGCCTTCCAATGAGGGGGATGAGCAACAAT ACCCCTCAGTTAAATCGCAGCTTATCACAAGGCACTCAGTTACCGAGCCACGTCACGCCAACAACAGGG GTACCAACAATGTCACTTCACACGCCTCCATCTCCAAGCAGGGGTATTTTGCCTATGAATCCTAGGAAT ATGATGAACCACTCCCAGGTTGGTCAGGGCATTGGAATTCCTAGCAGGACAAATAGCATGAGCAGTTCA GGGTTAGGTAGCCCCAACAGAAGCTCGCCAAGCATAATATGTATGCCAAAGCAGCAGCCTTCTCGACAG CCTTTTACTGTGAACAGTATGTCTGGATTTGGAATGAACAGGAATCAGGCATTTGGAATGAATAACTCC TTATCAAGTAACATTTTTAATGGAACAGACGGAAGTGAAAATGTGACAGGATTGGACCTTTCAGATTTC CCAGCATTAGCAGACCGAAACAGGAGGGAAGGAAGTGGTAACCCAACTCCATTAATAAACCCCTTGGCT GGAAGAGCTCCTTATGTTGGAATGGTAACAAAACCAGCAAATGAACAATCCCAGGACTTCTCAATACAC AATGAAGATTTTCCAGCATTACCAGGCTCCAGCTATAAAGATCCAACATCAAGTAATGATGACAGTAAA TCTAATTTGAATACATCTGGCAAGACAACTTCAAGTACAGATGGACCCAAATTCCCTGGAGATAAAAGT TCAACAACACAAAATAATAACCAGCAGAAAAAAGGGATCCAGGTGTTACCTGATGGTCGGGTTACTAAC ATTCCTCAAGGGATGGTGACGGACCAATTTGGAATGATTGGCCTGTTAACATTTATCAGGGCAGCAGAG ACAGACCCAGGAATGGTACATCTTGCATTAGGAAGTGACTTAACAACATTAGGCCTCAATCTGAACTCT CCTGAAAATCTCTACCCCAAATTTGCGTCACCCTGGGCATCTTCACCTTGTCGACCTCAAGACATAGAC TTCCATGTTCCATCTGAGTACTTAACGAACATTCACATTAGGGATAAGCTGGCTGCAATAAAACTTGGC CGATATGGTGAAGACCTTCTCTTCTATCTCTATTACATGAATGGAGGAGACGTATTACAACTTTTAGCT GCAGTGGAGCTTTTTAACCGTGATTGGAGATACCACAAAGAAGAACGAGTATGGATTACCAGGGCACCA GGCATGGAGCCAACAATGAAAACCAATACCTATGAGAGGGGAACATATTACTTCTTTGACTGTCTTAAC TGGAGGAAAGTAGCTAAGGAGTTCCATCTGGAATATGACAAATTAGAAGAACGGCCTCACCTGCCATCC ACCTTCAACTACAACCCTGCTCAGCAAGCCTTCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199302
Insert Size	1623 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199302.1
RefSeq Size	3162 bp
RefSeq ORF	1623 bp
Locus ID	4848
UniProt ID	Q9NZN8
Protein Families	Transcription Factors
Protein Pathways	RNA degradation
MW	59.7 kDa
Gene Summary	This gene encodes a subunit of the multi-component CCR4-NOT complex. The CCR4-NOT complex regulates mRNA synthesis and degradation and is also thought to be involved in mRNA splicing, transport and localization. The encoded protein interacts with histone deacetylases and functions as a repressor of polymerase II transcription. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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