NOS1 (NM_001204218) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331527 representing NM_001204218. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGGATCACATGTTCGGTGTTCAGCAAATCCAGCCCAATGTCATTTCTGTTCGTCTCTTCAAGCGC AAAGTTGGGGGCCTGGGATTTCTGGTGAAGGAGCGGGTCAGTAAGCCGCCCGTGATCATCTCTGACCTG ATTCGTGGGGGCGCCGCAGAGCAGAGTGGCCTCATCCAGGCCGGAGACATCATTCTTGCGGTCAACGGC CGGCCCTTGGTGGACCTGAGCTATGACAGCGCCCTGGAGGTACTCAGAGGCATTGCCTCTGAGACCCAC GTGGTCCTCATTCTGAGGGGCCCTGAAGGTTTCACCACGCACCTGGAGACCACCTTTACAGGTGATGGG ACCCCCAAGACCATCCGGGTGACACAGCCCCTGGGTCCCCCCACCAAAGCCGTGGATCTGTCCCACCAG CCACCGGCCGGCAAAGAACAGCCCCTGGCAGTGGATGGGGCCTCGGGTCCCGGGAATGGGCCTCAGCAT GCCTACGATGATGGGCAGGAGGCTGGCTCACTCCCCCATGCCAACGGCCTGGCCCCCAGGCCCCCAGGC CAGGACCCCGCGAAGAAAGCAACCAGAGTCAGCCTCCAAGGCAGAGGGGAGAACAATGAACTGCTCAAG GAGATAGAGCCTGTGCTGAGCCTTCTCACCAGTGGGAGCAGAGGGGTCAAGGGAGGGGCACCTGCCAAG GCAGAGATGAAAGATATGGGAATCCAGGTGGACAGAGATTTGGACGGCAAGTCACACAAACCTCTGCCC CTCGGCGTGGAGAACGACCGAGTCTTCAATGACCTATGGGGGAAGGGCAATGTGCCTGTCGTCCTCAAC AACCCATATTCAGAGAAGGAGCAGCCCCCCACCTCAGGAAAACAGTCCCCCACAAAGAATGGCAGCCCC TCCAAGTGTCCACGCTTCCTCAAGGTCAAGAACTGGGAGACTGAGGTGGTTCTCACTGACACCCTCCAC CTTAAGAGCACATTGGAAACGGGATGCACTGAGTACATCTGCATGGGCTCCATCATGCATCCTTCTCAG CATGCAAGGAGGCCTGAAGACGTCCGCACAAAAGGACAGCTCTTCCCTCTCGCCAAAGAGTTTATTGAT CAATACTATTCATCAATTAAAAGATTTGGCTCCAAAGCCCACATGGAAAGGCTGGAAGAGGTGAACAAA GAGATCGACACCACTAGCACTTACCAGCTCAAGGACACAGAGCTCATCTATGGGGCCAAGCACGCCTGG CGGAATGCCTCGCGCTGTGTGGGCAGGATCCAGTGGTCCAAGCTGCAGGTATTCGATGCCCGTGACTGC ACCACGGCCCACGGGATGTTCAACTACATCTGTAACCATGTCAAGTATGCCACCAACAAAGGGAACCTC AGGTCTGCCATCACCATATTCCCCCAGAGGACAGACGGCAAGCACGACTTCCGAGTCTGGAACTCCCAG CTCATCCGCTACGCTGGCTACAAGCAGCCTGACGGCTCCACCCTGGGGGACCCAGCCAATGTGCAGTTC ACAGAGATATGCATACAGCAGGGCTGGAAACCGCCTAGAGGCCGCTTCGATGTCCTGCCGCTCCTGCTT CAGGCCAACGGCAATGACCCTGAGCTCTTCCAGATTCCTCCAGAGCTGGTGTTGGAAGTTCCCATCAGG CACCCCAAGTTTGAGTGGTTCAAGGACCTGGGGCTGAAGTGGTACGGCCTCCCCGCCGTGTCCAACATG CTCCTAGAGATTGGCGGCCTGGAGTTCAGCGCCTGTCCCTTCAGTGGCTGGTACATGGGCACAGAGATT GGTGTCCGCGACTACTGTGACAACTCCCGCTACAATATCCTGGAGGAAGTGGCCAAGAAGATGAACTTA GACATGAGGAAGACGTCCTCCCTGTGGAAGGACCAGGCGCTGGTGGAGATCAATATCGCGGTTCTCTAT AGCTTCCAGAGTGACAAAGTGACCATTGTTGACCATCACTCCGCCACCGAGTCCTTCATTAAGCACATG GAGAATGAGTACCGCTGCCGGGGGGGCTGCCCTGCCGACTGGGTGTGGATCGTGCCCCCCATGTCCGGA AGCATCACCCCTGTGTTCCACCAGGAGATGCTCAACTACCGGCTCACCCCCTCCTTCGAATACCAGCCT GATCCCTGGAACACGCATGTCTGGAAAGGCACCAACGGGACCCCCACAAAGCGGCGAGCCATTGGCTTC AAGAAGCTAGCAGAAGCTGTCAAGTTCTCGGCCAAGCTGATGGGGCAGGCTATGGCCAAGAGGGTGAAA GCGACCATCCTCTATGCCACAGAGACAGGCAAATCGCAAGCTTATGCCAAGACCTTGTGTGAGATCTTC AAACACGCCTTTGATGCCAAGGTGATGTCCATGGAAGAATATGACATTGTGCACCTGGAACATGAAACT CTGGTCCTTGTGGTCACCAGCACCTTTGGCAATGGAGATCCCCCTGAGAATGGGGAGAAATTCGGCTGT GCTTTGATGGAAATGAGGCACCCCAACTCTGTGCAGGAAGAAAGGAAGTACCCGGAACCCTTGCGTTTC TTTCCCCGTAAAGGGCCTCCCCTCCCCAATGGTGACACAGAAGTCCACGGTCTGGCTGCAGCCCGTGAC AGCCAGCACAGGAGCTACAAGGTCCGATTCAACAGCGTCTCCTCCTACTCTGACTCCCAAAAATCATCA GGCGATGGGCCCGACCTCAGAGACAACTTTGAGAGTGCTGGACCCCTGGCCAATGTGAGGTTCTCAGTT TTTGGCCTCGGCTCACGAGCATACCCTCACTTTTGCGCCTTCGGACACGCTGTGGACACCCTCCTGGAA GAACTGGGAGGGGAGAGGATCCTGAAGATGAGGGAAGGGGATGAGCTCTGTGGGCAGGAAGAGGCTTTC AGGACCTGGGCCAAGAAGGTCTTCAAGGCAGCCTGTGATGTCTTCTGTGTGGGAGATGATGTCAACATT GAAAAGGCCAACAATTCCCTCATCAGCAATGATCGCAGCTGGAAGAGAAACAAGTTCCGCCTCACCTTT GTGGCCGAAGCTCCAGAACTCACACAAGGTCTATCCAATGTCCACAAAAAGCGAGTCTCAGCTGCCCGG CTCCTTAGCCGTCAAAACCTCCAGAGCCCTAAATCCAGTCGGTCAACTATCTTCGTGCGTCTCCACACC AACGGGAGCCAGGAGCTGCAGTACCAGCCTGGGGACCACCTGGGTGTCTTCCCTGGCAACCACGAGGAC CTCGTGAATGCCCTGATCGAGCGGCTGGAGGACGCGCCGCCTGTCAACCAGATGGTGAAAGTGGAACTG CTGGAGGAGCGGAACACGGCTTTAGGTGTCATCAGTAACTGGACAGACGAGCTCCGCCTCCCGCCCTGC ACCATCTTCCAGGCCTTCAAGTACTACCTGGACATCACCACGCCACCAACGCCTCTGCAGCTGCAGCAG TTTGCCTCCCTAGCTACCAGCGAGAAGGAGAAGCAGCGTCTGCTGGTCCTCAGCAAGGGTTTGCAGGAG TACGAGGAATGGAAATGGGGCAAGAACCCCACCATCGTGGAGGTGCTGGAGGAGTTCCCATCTATCCAG ATGCCGGCCACCCTGCTCCTGACCCAGCTGTCCCTGCTGCAGCCCCGCTACTATTCCATCAGCTCCTCC CCAGACATGTACCCTGATGAAGTGCACCTCACTGTGGCCATCGTTTCCTACCGCACTCGAGATGGAGAA GGACCAATTCACCACGGCGTATGCTCCTCCTGGCTCAACCGGATACAGGCTGACGAACTGGTCCCCTGT TTCGTGAGAGGAGCACCCAGCTTCCACCTGCCCCGGAACCCCCAAGTCCCCTGCATCCTCGTTGGACCA GGCACCGGCATTGCCCCTTTCCGAAGCTTCTGGCAACAGCGGCAATTTGATATCCAACACAAAGGAATG AACCCCTGCCCCATGGTCCTGGTCTTCGGGTGCCGGCAATCCAAGATAGATCATATCTACAGGGAAGAG ACCCTGCAGGCCAAGAACAAGGGGGTCTTCAGAGAGCTGTACACGGCTTACTCCCGGGAGCCAGACAAA CCAAAGAAGTACGTGCAGGACATCCTGCAGGAGCAGCTGGCGGAGTCTGTGTACCGAGCCCTGAAGGAG CAAGGGGGCCACATATACGTCTGTGGGGACGTCACCATGGCTGCTGATGTCCTCAAAGCCATCCAGCGC ATCATGACCCAGCAGGGGAAGCTCTCGGCAGAGGACGCCGGCGTATTCATCAGCCGGATGAGGGATGAC AACCGATACCATGAGGATATTTTTGGAGTCACCCTGCGAACGTACGAAGTGACCAACCGCCTTAGATCT GAGTCCATTGCCTTCATTGAAGAGAGCAAAAAAGACACCGATGAGGTTTTCAGCTCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001204218
Insert Size	4407 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204218.1
RefSeq Size	12291 bp
RefSeq ORF	4407 bp
Locus ID	4842
UniProt ID	P29475
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer
MW	164.8 kDa
Gene Summary	The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011] Transcript Variant: This variant (2) contains an additional in-frame coding exon compared to variant 1, resulting in a longer isoform (2, also known as nNOSmu) containing a 34 aa protein segment not found in isoform 1. A similar isoform has also been reported in rat and mouse, and the mouse isoform has been shown to have catalytic activity (PMID:9791007). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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