PDLIM5 (NM_001256426) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ENH; ENH1; L9; LIM
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332381 representing NM_001256426. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGCAACTACAGTGTGTCACTGGTTGGCCCAGCTCCTTGGGGTTTCCGGCTGCAGGGCGGTAAGGAT TTCAACATGCCTCTGACAATCTCTAGTCTAAAAGATGGCGGCAAGGCAGCCCAGGCAAATGTAAGAATA GGCGATGTGGTTCTCAGCATTGATGGAATAAATGCACAAGGAATGACTCATCTTGAAGCCCAGAATAAG ATTAAGGGTTGTACAGGCTCTTTGAATATGACTCTGCAAAGAGCATCTGCTGCACCCAAGCCTGAGCCG GTTCCTGTTCAAAAGCCCACAGTCACCAGCGTGTGTTCCGAGACTTCTCAGGAGCTAGCAGAGGGACAG AGAAGAGGATCCCAGGGTGACAGTAAACAGCAAAATGGGAAAATCCCACCTAAACGCCCACCAAGAAAA CACATTGTGGAGCGCTATACAGAGTTTTATCATGTACCCACTCACAGTGATGCCAGCAAGAAGAGACTG ATTGAGGATACTGAAGACTGGCGTCCAAGGACTGGAACAACTCAGTCTCGCTCTTTCCGAATCCTTGCC CAGATCACTGGGACTGAACATTTGAAAGAATCTGAAGCCGATAATACAAAGAAGGCAAAGTTTGACAGT GCTCTGGAAGACCTACCTAAGAGTGGACCTCACCCTCCTGCAACTCCTCAGGTGTTAACTATTGGTAGC CAAGTGGCCACACTTTCTAAAGTAGCAACTACTTATTCTAGTTTAAGCAGCTCCACAGGAAATGTGGAA GATTCTTTCGAAGGTTTTCGAAACTTTTCTACCTTCTCTTCTCCTGCTAGATATAGTGCTGCAGTTCTG AGCAGCGCAGCTGCTACTGTGTCTGCTGTTATTGCTACAAAAACCAGGCTCTACACTCCTGAAAGATAT CACTCACTCCTGGATGCACTTTGCATCAGCCCTGTCTCCAAGCCTTTAGCTTTTTCCTATCTGCAGTCC TCAAGGAAATCAACTGGCTCTATCCATGTTAAGAAAACAAGTAACTCTCAGGAGCCTTCTCCGCAGTTG GCTTCCTCGGTAGCTTCCACACGGAGCATGCCCGAGAGCCTGGACAGCCCAACCTCTGGCAGACCAGGG GTTACCAGCCTCACAACTGCAGCTGCCTTCAAGCCTGTAGGATCCACTGGCGTCATCAAGTCACCAAGC TGGCAACGGCCAAACCAAGGAGTACCTTCCACTGGAAGAATCTCAAACAGCGCTACTTACTCAGGATCA GTGGCACCAGCCAACTCAGCTTTGGGACAAACCCAGCCAAGTGACCAGGACACTTTAGTGCAAAGAGCT GAGCACATTCCAGCAGGGAAACGAACTCCGATGTGCGCCCATTGTAACCAGGTCATCAGAGGACCATTC TTAGTGGCACTGGGGAAATCTTGGCACCCAGAAGAATTCAACTGCGCTCACTGCAAAAATACAATGGCC TACATTGGATTTGTAGAGGAGAAAGGAGCCCTGTATTGTGAGCTGTGCTATGAGAAATTCTTTGCCCCT GAATGTGGTCGATGCCAAAGGAAGATCCTTGGAGAAGTCATCAGTGCGTTGAAACAAACTTGGCATGTT TCCTGTTTTGTGTGTGTAGCCTGTGGAAAGCCCATTCGGAACAATGTTTTTCACTTGGAGGATGGTGAA CCCTACTGTGAGACTGATTATTATGCCCTCTTTGGTACTATATGCCATGGATGTGAATTTCCCATAGAA GCTGGTGACATGTTCCTGGAAGCTCTGGGCTACACCTGGCATGACACTTGCTTTGTATGCTCAGTGTGT TGTGAAAGTTTGGAAGGTCAGACCTTTTTCTCCAAGAAGGACAAGCCCCTGTGTAAGAAACATGCTCAT TCTGTGAATTTTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256426
Insert Size	1878 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256426.1
RefSeq Size	6219 bp
RefSeq ORF	1878 bp
Locus ID	10611
Protein Families	Druggable Genome
MW	67.8 kDa
Gene Summary	This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (6) uses an alternate in-frame splice site in the 5' coding region, and contains four additional in-frame exons in the central coding region, compared to variant 1, resulting in an isoform (f) that is longer than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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