Pirh2 (RCHY1) (NM_001278537) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY; ZNF363
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001278537, the custom clone sequence may differ by one or more nucleotides ATGGCGGCGACGGCCCGGGAAGATGGCGCCAGCGGTCAAGAGCGAGGTCAGCGGGGCTGCGAGCACTATG ACAGAGGATGTCTCCTAAAGGCCCAACAGACTTGTGAAGAATGTAGCACATTGTTTGGAGAATATTATTG CGATATATGCCATTTGTTTGACAAAGATAAGAAGCAGTATCACTGTGAAAACTGTGGAATTTGTAGGATT GGTCCAAAGGAAGATTTTTTCCATTGTTTGAAATGTAACTTATGCCTAGCTATGAATCTTCAAGGAAGAC ACAAGTGTATTGAAAATGTGTCCCGACAGAATTGTCCAATATGTTTGGAGGACATTCACACATCCCGTGT TGTTGCTCATGTCTTGCCATGTGGACATCTTTTACATAGAGGCTACAGATGTCCATTATGTATGCACTCT GCTTTAGATATGACCAGGTATTGGAGACAGCTGGATGATGAAGTAGCACAGACTCCTATGCCATCAGAAT ATCAGAACATGACTGTGGATATTCTCTGCAATGACTGTAATGGACGATCCACTGTTCAGTTTCATATATT AGGCATGAAATGTAAGATTTGTGAATCCTATAATACTGCTCAAGCTGGAGGACGTAGAATTTCACTGGAT CAGCAATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001278537
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278537.1, NP_001265466.1
RefSeq Size	4284 bp
RefSeq ORF	639 bp
Locus ID	25898
UniProt ID	Q96PM5
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	p53 signaling pathway, Ubiquitin mediated proteolysis
Gene Summary	The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2013] Transcript Variant: This variant (7) lacks two in-frame coding exons, compared to variant 1. The encoded isoform (5) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.