ACADS (NM_001302554) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACAD3; SCAD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335920 representing NM_001302554. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCGCCGCGCTGCTCGCCCGGGCCTCGGGCCCTGCCCGCAGAGCTCTCTGTCCTAGGGCCTGGCGG CAGTTACACACCATCTACCAGTCTGTGGAACTGCCCGAGACACACCAGATGTTGCTCCAGACATGCCGG GACTTTGCCGAGAAGGAGTTGTTTCCCATTGCAGCCCAGGTGGATAAGGAACATCTCTTCCCAGCGGCT CAGGTGAAGAAGATGGGCGGGCTTGGGCTTCTGGCCATGGACGTGCCCGAGGAGCTTGGCGGTGCTGGC CTCGATTACCTGGCCTACGCCATCGCCATGGAGGAGATCAGCCGTGGCTGCGCCTCCACCGGAGTCATC ATGAGTGTCAACAACTCTCTCTACCTGGGGCCCATCTTGAAGTTTGGCTCCAAGGAGCAGAAGCAGGCG TGGGTCACGCCTTTCACCAGTGGTGACAAAATTGGCTGCTTTGCCCTCAGCGAACCAGGGCCTTCTCTC CTTGGCCCGACTGGACCTATTTTTGCTCTGGGGCAAGTGGGCTGTCCCTGTCCCTCCTCAGCTGCCACT GAAGCCTGCACCTTCCCCAGAAGCCGGCAGAGGGTGTCAAGGCCTGAGCTTCTGAGGGAGGGCATCAGT GCCTTCCTGGTCCCCATGCCAACGCCTGGGCTCACGTTGGGGAAGAAAGAAGACAAGCTGGGCATCCGG GGCTCATCCACGGCCAACCTCATCTTTGAGGACTGTCGCATCCCCAAGGACAGCATCCTGGGGGAGCCA GGGATGGGCTTCAAGATAGCCATGCAAACCCTGGACATGGGCCGCATCGGCATCGCCTCCCAGGCCCTG GGCATTGCCCAGACCGCCCTCGATTGTGCTGTGAACTACGCTGAGAATCGCATGGCCTTCGGGGCGCCC CTCACCAAGCTCCAGGTCATCCAGTTCAAGTTGGCAGACATGGCCCTGGCCCTGGAGAGTGCCCGGCTG CTGACCTGGCGCGCTGCCATGCTGAAGGATAACAAGAAGCCTTTCATCAAGGAGGCAGCCATGGCCAAG CTGGCCGCCTCGGAGGCCGCGACCGCCATCAGCCACCAGGCCATCCAGATCCTGGGCGGCATGGGCTAC GTGACAGAGATGCCGGCAGAGCGGCACTACCGCGACGCCCGCATCACTGAGATCTACGAGGGCACCAGC GAAATCCAGCGGCTGGTGATCGCCGGGCATCTGCTCAGGAGCTACCGGAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001302554
Insert Size	1227 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302554.1
RefSeq Size	1952 bp
RefSeq ORF	1227 bp
Locus ID	35
UniProt ID	P16219
Protein Families	Druggable Genome
Protein Pathways	Butanoate metabolism, Fatty acid metabolism, Metabolic pathways, Valine, leucine and isoleucine degradation
MW	44 kDa
Gene Summary	This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (2) lacks an alternate exon and contains an alternate exon in the 3' coding region but maintains the reading frame. It encodes isoform 2 which is shorter compared to isoform 1.

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