TAP1 (NM_001292022) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ABC17; ABCB2; APT1; D6S114E; PSF-1; PSF1; RING4; TAP1*0102N; TAP1N
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336735 representing NM_001292022. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCATTCCATTCTTTACGGGCCGCCTCACTGACTGGATTCTACAAGATGGCTCAGCCGATACCTTC ACTCGAAACTTAACTCTCATGTCCATTCTCACCATAGCCAGTGCAGTGCTGGAGTTCGTGGGTGACGGG ATCTATAACAACACCATGGGCCACGTGCACAGCCACTTGCAGGGAGAGGTGTTTGGGGCTGTCCTGCGC CAGGAGACGGAGTTTTTCCAACAGAACCAGACAGGTAACATCATGTCTCGGGTAACAGAGGACACGTCC ACCCTGAGTGATTCTCTGAGTGAGAATCTGAGCTTATTTCTGTGGTACCTGGTGCGAGGCCTATGTCTC TTGGGGATCATGCTCTGGGGATCAGTGTCCCTCACCATGGTCACCCTGATCACCCTGCCTCTGCTTTTC CTTCTGCCCAAGAAGGTGGGAAAATGGTACCAGTTGCTGGAAGTGCAGGTGCGGGAATCTCTGGCAAAG TCCAGCCAGGTGGCCATTGAGGCTCTGTCGGCCATGCCTACAGTTCGAAGCTTTGCCAACGAGGAGGGC GAAGCCCAGAAGTTTAGGGAAAAGCTGCAAGAAATAAAGACACTCAACCAGAAGGAGGCTGTGGCCTAT GCAGTCAACTCCTGGACCACTAGTATTTCAGGTATGCTGCTGAAAGTGGGAATCCTCTACATTGGTGGG CAGCTGGTGACCAGTGGGGCTGTAAGCAGTGGGAACCTTGTCACATTTGTTCTCTACCAGATGCAGTTC ACCCAGGCTGTGGAGGTACTGCTCTCCATCTACCCCAGAGTACAGAAGGCTGTGGGCTCCTCAGAGAAA ATATTTGAGTACCTGGACCGCACCCCTCGCTGCCCACCCAGTGGTCTGTTGACTCCCTTACACTTGGAG GGCCTTGTCCAGTTCCAAGATGTCTCCTTTGCCTACCCAAACCGCCCAGATGTCTTAGTGCTACAGGGG CTGACATTCACCCTACGCCCTGGCGAGGTGACGGCGCTGGTGGGACCCAATGGGTCTGGGAAGAGCACA GTGGCTGCCCTGCTGCAGAATCTGTACCAGCCCACCGGGGGACAGCTGCTGTTGGATGGGAAGCCCCTT CCCCAATATGAGCACCGCTACCTGCACAGGCAGGTGGCTGCAGTGGGACAAGAGCCACAGGTATTTGGA AGAAGTCTTCAAGAAAATATTGCCTATGGCCTGACCCAGAAGCCAACTATGGAGGAAATCACAGCTGCT GCAGTAAAGTCTGGGGCCCATAGTTTCATCTCTGGACTCCCTCAGGGCTATGACACAGAGGTAGACGAG GCTGGGAGCCAGCTGTCAGGGGGTCAGCGACAGGCAGTGGCGTTGGCCCGAGCATTGATCCGGAAACCG TGTGTACTTATCCTGGATGATGCCACCAGTGCCCTGGATGCAAACAGCCAGTTACAGGTGGAGCAGCTC CTGTACGAAAGCCCTGAGCGGTACTCCCGCTCAGTGCTTCTCATCACCCAGCACCTCAGCCTGGTGGAG CAGGCTGACCACATCCTCTTTCTGGAAGGAGGCGCTATCCGGGAGGGGGGAACCCACCAGCAGCTCATG GAGAAAAAGGGGTGCTACTGGGCCATGGTGCAGGCTCCTGCAGATGCTCCAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001292022
Insert Size	1644 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001292022.1
RefSeq Size	2234 bp
RefSeq ORF	1644 bp
Locus ID	6890
UniProt ID	Q03518
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	ABC transporters, Antigen processing and presentation, Primary immunodeficiency
MW	60 kDa
Gene Summary	The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.

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