FUBP1 (NM_001303433) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FBP; FUBP; hDH V
Vector	PCMV6-Neo
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001303433, the custom clone sequence may differ by one or more nucleotides ATGGCAGACTATTCAACAGTGCCTCCCCCCTCTTCTGGCTCAGCTGGTGGCGGTGGTGGCGGCGGTGGTG GTGGAGGAGTTAACGACGCTTTCAAAGATGCACTGCAGAGAGCCCGGCAGATTGCAGCAAAAATTGGAGG TGATGCAGGGACATCACTGAATTCAAATGACTATGGTTATGGGGGACAAAAAAGACCTTTAGAAGATGGA GATGGCTCTTGGACAAGTCCGAGCAGTACAACACACTGGGAGGGAATGCCCTCTCCTTTTAAAGATCAAC CAGATGCTAAGAAAGTTGCTCCTCAAAATGACTCTTTTGGAACACAGTTACCACCGATGCATCAGCAGCA AAGCAGATCTGTAATGACAGAAGAATACAAAGTTCCAGATGGAATGGTTGGATTCATAATTGGCAGAGGA GGTGAACAGATCTCACGCATACAACAGGAATCTGGATGCAAAATACAGATAGCTCCTGACAGTGGTGGCC TTCCAGAAAGGTCCTGTATGTTAACTGGAACACCTGAATCTGTCCAGTCAGCAAAACGGTTACTGGACCA GATTGTTGAAAAAGGAAGACCAGCTCCTGGCTTCCATCATGGCGATGGACCGGGAAATGCAGTTCAAGAA ATCATGATTCCAGCTAGCAAGGCAGGATTAGTCATTGGAAAAGGGGGAGAAACTATTAAACAGCTTCAGG AACGGGCTGGAGTTAAAATGGTTATGATTCAAGACGGGCCGCAGAACACTGGTGCTGACAAACCTCTTAG GATTACAGGAGACCCATATAAAGTTCAACAAGCCAAGGAAATGGTGTTAGAGTTAATTCGTGATCAAGGC GGTTTCAGAGAAGTTCGGAATGAGTATGGGTCAAGAATAGGAGGAAATGAAGGGATAGATGTCCCCATTC CAAGATTTGCTGTTGGCATTGTAATAGGAAGAAATGGAGAGATGATCAAAAAAATACAAAATGATGCTGG TGTTCGCATTCAGTTTAAGCCAGATGATGGGACAACACCCGAAAGGATAGCACAAATAACAGGACCTCCA GACCGATGTCAACATGCTGCAGAAATTATTACAGACCTTCTTCGAAGTGTTCAGGCTGGTAATCCTGGTG GACCTGGACCTGGTGGTCGAGGAAGAGGTAGAGGTCAAGGCAACTGGAACATGGGACCACCTGGTGGACT ACAGGAATTTAATTTTATTGTGCCAACTGGGAAAACTGGATTAATAATAGGAAAAGGAGGTGAAACCATA AAAAGCATAAGCCAGCAGTCTGGTGCAAGAATAGAACTTCAGAGAAATCCTCCACCAAATGCAGATCCTA ATATGAAGTTATTTACAATTCGTGGCACTCCACAACAGATAGACTATGCTCGGCAACTCATAGAAGAAAA GATTGGTGGCCCAGTAAATCCTTTAGGGCCACCTGTACCCCATGGGCCCCATGGTGTCCCAGGCCCCCAT GGACCTCCTGGGCCTCCAGGGCCTGGAACTCCAATGGGACCATACAACCCTGCACCTTATAATCCTGGAC CACCAGGCCCGGCTCCTCATGGTCCTCCAGCCCCATATGCTCCCCAGGGATGGGGAAATGCATATCCACA CTGGCAGCAGCAGGCTCCTCCTGATCCAGCTAAGGCAGGAACGGATCCAAATTCAGCAGCTTGGGCTGCT TATTACGCTCACTATTATCAACAGCAAGCACAGCCACCACCAGCAGCCCCTGCAGGTGCACCAACTACAA CTCAAACTAATGGACAAGGAGATCAGCAGAATCCAGCCCCAGCTGGACAGGTTGATTATACCAAGGCTTG GGAAGAGTACTACAAGAAAATGGGTCAGGCAGTTCCTGCTCCGACTGGGGCTCCTCCAGGTGGTCAGCCA GATTATAGTGCAGCCTGGGCTGAGTATTATAGACAACAAGCAGCCTATTATGCCCAGACAAGTCCCCAGG GAATGCCACAGCATCCTCCAGCACCTCAGGGCCAATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001303433
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303433.1, NP_001290362.1
RefSeq Size	2585 bp
RefSeq ORF	1998 bp
Locus ID	8880
UniProt ID	Q96AE4
Protein Families	Stem cell - Pluripotency, Transcription Factors
Gene Summary	The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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