TRIP12 (NM_001284214) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MRD49; TRIP-12; TRIPC; ULF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001284214, the custom clone sequence may differ by one or more nucleotides ATGTCCAACCGGCCTAATAACAATCCAGGGGGGTCACTGCGACGTTCACAGAGGAACACTGCCGGGGCCC AACCACAAGACGACTCAATAGGAGGAAGGTCACATTTAGGGCAGGCAAAACATAAGGGATATAGCCCTCC TGAGAGTAGAAAATCTAATTCTAAGGCACCCAAAGTGCAGTCTAATACTACTTCTGAACTGTCAAGGGGA CACCTTTCTAAAAGAAGCTGCAGTTCATCATCTGCTGTGATAGTTCCACAACCAGAGGATCCAGACAGAG CCAATACTTCAGAAAGACAAAAAACGGGGCAGGTGCCTAAGAAAGACAATTCTCGAGGAGTGAAGCGCAG TGCTAGTCCAGACTACAACAGGACCAATTCTCCTAGCTCTGCAAAAAAACCAAAAGCACTTCAGCATACT GAATCTCCCTCAGAAACAAATAAGCCACATAGTAAGTCAAAGAAGAGACATTTAGACCAGGAGCAACAAC TGAAATCTGCACAATCACCATCAACAAGCAAGGCTCATACCAGGAAGAGTGGGGCCACTGGCGGTTCACG GAGTCAGAAAAGAAAAAGGACAGAGAGTTCTTGTGTAAAGAGTGGCTCCGGGTCTGAATCAACTGGTGCA GAAGAGAGATCTGCGAAACCTACCAAGCTGGCTTCAAAATCAGCCACCTCAGCCAAAGCTGGGTGTAGCA CCATCACTGATTCTTCTTCTGCTGCCTCTACTTCCTCCTCGTCTTCTGCTGTAGCCTCGGCCTCCTCCAC TGTACCACCAGGTGCCAGAGTGAAACAAGGAAAAGATCAGAACAAGGCCAGGCGTTCCCGTTCAGCGTCC AGTCCCAGCCCCAGAAGAAGTAGCAGGGAAAAGGAACAGAGTAAAACTGGTGGCTCTTCAAAATTTGATT GGGCTGCTCGTTTCAGCCCTAAAGTTAGCCTTCCTAAAACAAAACTGTCTCTTCCAGGGTCTTCTAAGTC AGAGACATCAAAACCTGGACCTTCTGGATTACAGGCCAAATTAGCAAGTTTAAGAAAATCTACGAAGAAA CGCAGTGAGTCTCCACCTGCTGAGCTCCCCAGTTTGAGGCGGAGCACACGCCAAAAGACCACGGGCTCCT GTGCTAGTACCAGTCGGCGAGGCTCTGGCCTGGGCAAAAGAGGAGCAGCTGAAGCTCGTCGACAGGAGAA AATGGCAGACCCTGAAAGCAACCAGGAGGCAGTAAATTCTTCAGCTGCTCGGACAGATGAAGCTCCCCAA GGAGCTGCAGCCTCTAGTTCTGTTGCAGGGGCTGTTGGCATGACCACCTCTGGGGAGAGTGAATCAGATG ATTCCGAGATGGGACGTTTGCAAGCTTTGTTAGAGGCAAGGGGTCTTCCCCCTCACCTATTTGGTCCTCT TGGTCCTCGGATGTCACAGCTTTTCCATAGAACAATTGGAAGTGGAGCTAGTTCTAAGGCCCAGCAGCTA CTACAAGGATTGCAAGCCAGTGATGAAAGTCAACAGCTTCAGGCAGTTATTGAGATGTGTCAGTTACTGG TCATGGGAAATGAGGAGACACTGGGAGGGTTTCCTGTCAAGAGTGTTGTTCCAGCTTTGATTACGTTACT TCAGATGGAGCACAATTTTGATATTATGAACCATGCTTGTCGAGCCTTAACATACATGATGGAAGCACTT CCTCGATCTTCTGCTGTTGTAGTAGATGCTATTCCTGTCTTTTTAGAAAAGCTGCAAGTTATTCAGTGTA TTGATGTGGCAGAGCAGGCCTTGACTGCCTTGGAGATGTTGTCACGGAGACATAGTAAAGCCATTCTACA GGCGGGTGGTTTGGCAGACTGCTTGCTGTACCTAGAATTCTTCAGCATAAATGCCCAAAGAAATGCATTA GCAATTGCAGCTAATTGCTGCCAGAGTATCACGCCAGATGAATTTCATTTTGTGGCAGATTCACTCCCAT TGCTAACCCAAAGGCTAACACATCAGGATAAAAAGTCAGTAGAAAGCACTTGCCTTTGTTTTGCACGCCT AGTGGACAACTTCCAGCATGAGGAGAATTTACTCCAGCAGGTTGCTTCCAAAGATCTGCTTACAAATGTT CAACAGCTGTTGGTAGTGACTCCACCCATTTTAAGTTCTGGGATGTTTATAATGGTGGTTCGCATGTTTT CTCTGATGTGTTCCAACTGTCCAACTTTAGCTGTTCAACTTATGAAACAAAACATTGCAGAAACGCTTCA CTTTCTCCTGTGTGGTGCCTCCAATGGAAGTTGTCAGGAACAGATTGATCTTGTTCCACGAAGCCCTCAA GAGTTGTATGAACTGACATCTCTGATTTGTGAACTTATGCCATGTTTACCAAAAGAAGGCATTTTTGCAG TTGATACCATGTTGAAGAAGGGAAATGCACAGAACACAGATGGTGCGATATGGCAGTGGCGTGATGATCG GGGCCTCTGGCATCCATATAACAGGATTGACAGCCGGATCATTGAGCAAATCAATGAGGACACGGGAACA GCACGTGCCATTCAGAGAAAACCTAACCCGTTAGCCAATAGTAACACTAGTGGATATTCAGAGTCAAAGA AGGATGATGCTCGAGCACAGCTTATGAAAGAGGATCCGGAACTGGCTAAGTCTTTTATTAAGACATTATT TGGTGTTCTTTATGAAGTGTATAGTTCCTCAGCAGGACCTGCGGTCAGACATAAGTGCCTTAGAGCAATT CTTAGGATAATTTATTTTGCGGATGCTGAACTTCTGAAGGATGTTCTGAAAAATCATGCTGTTTCAAGTC ACATTGCTTCCATGCTGTCAAGCCAAGACCTGAAGATAGTAGTGGGAGCACTTCAGATGGCAGAAATTTT AATGCAGAAGTTACCTGATATTTTTAGTGTTTACTTCAGAAGAGAAGGTGTAATGCATCAAGTAAAACAC TTAGCAGAATCAGAGTCTTTGTTGACAAGTCCACCAAAGGCATGTACGAATGGATCGGGATCCATGGGAT CCACAACTTCAGTCAGCAGTGGGACAGCCACAGCTGCCACTCATGCTGCAGCTGACTTGGGATCACCCAG CTTGCAGCACAGCAGGGATGATTCTTTAGATCTCAGCCCTCAAGGTCGATTAAGTGATGTTCTAAAGAGA AAACGACTGCCAAAACGAGGGCCAAGAAGGCCAAAGTACTCACCTCCAAGAGATGATGACAAAGTAGACA ATCAAGCTAAAAGCCCCACCACTACTCAGTCACCTAAATCTTCTTTCCTGGCAAGCTTGAATCCAAAAAC ATGGGGAAGGTTAAGTACACAGTCCAACAGCAACAACATTGAGCCAGCACGGACTGCGGGAGGTAGTGGC CTTGCCAGGGCTGCCTCAAAGGATACCATCTCCAATAATAGAGAAAAAATTAAAGGTTGGATTAAGGAGC AGGCACATAAATTTGTAGAACGTTATTTCAGTTCTGAGAATATGGATGGAAGCAACCCTGCATTGAATGT CCTTCAGAGACTTTGTGCTGCAACCGAACAACTCAACCTCCAGGTGGATGGTGGAGCTGAGTGCCTTGTA GAAATCCGTAGCATAGTCTCAGAGTCAGATGTTTCATCATTTGAAATCCAACATAGTGGATTTGTGAAGC AGCTGTTGCTTTATTTGACATCTAAAAGTGAAAAGGATGCTGTGAGCAGAGAGATCAGATTAAAGCGATT TCTTCATGTATTTTTTTCTTCTCCACTTCCTGGAGAAGAGCCCATTGGAAGAGTGGAACCAGTGGGTAAT GCACCTTTGTTGGCATTAGTTCACAAGATGAACAACTGCCTCAGCCAGATGGAACAATTTCCAGTCAAAG TACATGATTTCCCTAGTGGAAATGGGACAGGAGGCAGCTTTTCTCTCAACAGAGGATCACAGGCTTTAAA ATTTTTCAACACACATCAATTAAAATGCCAGTTACAAAGGCATCCAGACTGTGCAAATGTGAAGCAGTGG AAGGGTGGACCTGTCAAGATTGACCCTCTGGCTTTGGTACAAGCCATCGAGAGATACCTTGTAGTTAGAG GGTATGGAAGAGTAAGAGAAGATGATGAAGACAGCGATGACGATGGATCAGATGAGGAAATAGATGAGTC TCTGGCTGCTCAGTTCCTAAATTCAGGAAATGTAAGACACAGGCTGCAGTTTTATATTGGAGAACATTTG CTGCCGTATAACATGACTGTGTATCAGGCAGTACGGCAGTTTAGTATACAGGCTGAAGATGAAAGAGAAT CCACAGATGATGAGAGCAATCCTCTAGGCAGAGCTGGTATTTGGACAAAGACTCATACAATATGGTATAA ACCTGTGAGAGAGGATGAAGAAAGTAATAAAGATTGTGTTGGTGGTAAAAGAGGAAGAGCCCAAACAGCT CCAACGAAAACTTCCCCTAGAAATGCAAAAAAGCATGATGAGTTATGGCACGATGGAGTGTGCCCATCAG TATCAAATCCTTTAGAAGTTTACCTCATTCCCACACCACCTGAAAATATAACATTTGAAGACCCGTCATT AGATGTGATCCTTCTTTTAAGAGTTTTACATGCTATCAGTCGATACTGGTATTACTTGTATGATAATGCA ATGTGCAAGGAAATTATTCCAACTAGTGAATTTATTAACAGTAAGTTAACAGCAAAAGCAAATAGGCAAC TTCAAGATCCTTTAGTAATCATGACAGGAAACATCCCAACATGGCTTACTGAGCTAGGAAAAACCTGCCC ATTTTTCTTTCCTTTTGATACCCGGCAAATGCTTTTTTATGTAACTGCATTTGATCGGGACCGAGCAATG CAAAGATTACTTGATACCAACCCAGAAATCAACCAGTCTGATTCTCAAGATAGCAGAGTTGCACCTAGAT TGGATAGAAAAAAACGTACTGTGAACCGAGAGGAGCTGCTGAAACAGGCGGAGTCTGTGATGCAGGACCT CGGCAGCTCACGGGCCATGTTAGAAATCCAGTATGAAAATGAGGTTGGTACAGGTCTTGGGCCTACACTG GAGTTTTATGCGCTTGTATCTCAGGAACTACAGAGAGCTGACTTGGGTCTTTGGAGAGGTGAAGAAGTAA CTCTTAGCAATCCAAAAGGGAGCCAAGAAGGGACCAAGTATATTCAAAACCTCCAGGGCCTGTTTGCGCT TCCCTTTGGTAGGACAGCAAAGCCAGCTCATATCGCAAAGGTTAAGATGAAGTTTCGCTTCTTAGGAAAA TTAATGGCCAAGGCTATCATGGATTTCAGATTGGTGGACCTTCCCCTTGGCTTACCCTTTTATAAATGGA TGCTACGGCAAGAAACTTCACTGACATCACACGATTTGTTTGACATCGACCCAGTTGTAGCCAGATCAGT TTATCACCTAGAAGACATTGTCAGACAGAAGAAAAGACTTGAACAAGATAAATCCCAGACCAAAGAGAGT CTACAGTATGCATTAGAAACCTTGACTATGAATGGCTGCTCAGTTGAAGATCTAGGACTGGATTTCACTC TGCCAGGGTTTCCCAATATCGAACTGAAGAAAGGAGGGAAGGATATACCAGTCACTATCCACAATTTAGA GGAGTATCTAAGACTGGTTATATTCTGGGCACTAAATGAAGGCGTTTCTAGGCAATTTGATTCGTTCAGA GATGGATTTGAATCAGTCTTCCCACTCAGTCATCTTCAGTACTTCTACCCGGAGGAACTGGATCAGCTCC TTTGTGGCAGTAAAGCAGACACTTGGGATGCAAAGACACTGATGGAATGCTGTAGGCCTGATCATGGTTA TACTCATGACAGTCGGGCTGTGAAGTTTTTGTTTGAGATTCTCAGTAGTTTTGATAATGAGCAGCAGAGG TTATTTCTCCAGTTTGTGACTGGTAGCCCAAGATTGCCTGTTGGAGGATTCCGGAGTTTGAATCCACCTT TGACAATTGTCCGAAAGACGTTTGAATCAACAGAAAACCCAGATGACTTCTTGCCCTCTGTAATGACTTG TGTGAACTATCTTAAGTTGCCGGACTATTCAAGCATTGAGATAATGCGTGAAAAACTGTTGATAGCAGCA AGAGAAGGGCAGCAGTCGTTCCATCTTTCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001284214
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284214.1, NP_001271143.1
RefSeq Size	10036 bp
RefSeq ORF	6123 bp
Locus ID	9320
UniProt ID	Q14669
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
Gene Summary	The protein encoded by this gene is an E3 ubiquitin-protein ligase involved in the degradation of the p19ARF/ARF isoform of CDKN2A, a tumor suppressor. The encoded protein also plays a role in the DNA damage response by regulating the stability of USP7, which regulates tumor suppressor p53. [provided by RefSeq, Jan 2017] Transcript Variant: This variant (1), as well as variant 5, encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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