pol (AC_000019) Virus Tagged ORF Clone

CAT＃: VC100078

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for pol [Human adenovirus 35], codon optimized for human cell expression, AP_000576

Datasheet

SDS

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CNY 18,050.00

货期*

7周

规格

Product images

经常一起买 (4)

PS100001

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TA592569

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【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100078 represents NCBI reference of AP_000576 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTCGTACAGGCGCACGGGGCCGGTTCCTTCCACCCCGAGACAAGCGACACTCGGAGGCAATCCA CGCGGGGAGGCGCGAGAAAATCTTCACAGAGTGCAATTCCAGAGCCGGCCCGCGCTAGACGCCGCCGCGC GACCACAAGTCATGCCCCAGGCAGTGGTACTACCGCTGCCGCTCGAAGAGCCAGCCCCTCACCTCTTCTG TCAATGCAGGAAGCCCGCGAGCCCCCCCCAATCAAGCGGAAGAACAAGGGCACCGTTGTCGCTCCCAAGG GTCACGGCACACTCCAGGCTATCGACGTGTCCACTAATGAGCCCGTGGAGATCAAATACCACCTGAACCT TCCTCACGCCCTGGAAAAGATTATGCAGGTCAATCTGCTGTCTCTTCCCACAAATCTGTCCCCCCAGCGC CTCCGCACCCTGGATAGTAGTAGCCTTCGGGCACTGGTTCTTCAGCTCCGGCCTTCCAGGGCTGAGGTTT GGACATGCCTTCCACGGGGACTCGTGAGCATGACGACAATTGAGACCGAGGACGAGCAGGCCGACACTGC GAATATTGAGGAACATGAGATTCAGTCCCCAGGACTCGGTTTCCCCCTGAAGTTCCTGGTGAAGGGTTCC CAAGTCCAGCTTATTCACGAGATACAGCCTGTGCAGAGATGTGACTACTGCGGCAGATTGTACAAACATA AACATGAGTGCTCTGCACGGCGGCGGAATTTTTACTTTCATCACATCAATTCCCAGTCAAGTAACTGGTG GCAGGAAATACAGTTTTTCCCCATCGGGTCCCACCCAAGGACGGAAAGACTCTTCTTGACATATGATGTG GAGACTTACACGTGGATGGGCTCCTTCGGGAAGCAGCTTGTCCCCTTCATGTTGGTCATGAAATTGTCTG GAGAAGAGCGACTCGTCAACATGGCACACGACCTTGCAATAAAGCTTAAATGGGATAGGTGGCAGCAGGA TCCGCGGACCTTCTACTGTGTTACGCCGGAGAAGATGGCTATTGGACGCCACTTTCGCCAGTATCGGGAT CAGCTGCAGACTGCGTTGGCCGTCGATCTCTGGTCATCATTTCTGCACGCCAACCCACACCTGCATGAAT GGGCGCTGGAACACTACGCCCTGAGCGACCCTACGGATCTGACTTTCGATGAACTCAGTAAGCTGCCTCA CGTGCGGGGCACCCCACGATTTATCGAGCTGTACATTGTCGGACACAATATCAATGGATTTGATGAAATT GTCCTCGCAGCACAAGTGATAAATAATCGCGCAGAGGTGCCTCAGCCTTTCCGCATTACTAGAAACTTTA TGCCTAGAGCTGGGAAAATTCTGTTTAATGATGTGACGTTCGCTCTGCCCAACCCCGCCTACAAAAAGAG AGTCGATTTCCAGCTGTGGGAGCAAGGCGCATGTGACGATATGGACTTCAAATACCAGTTTCTGAAAGTT ATGGTAAGGGATACGTTCGCACTCACCCATGCCTCACTGAGGAAGGCTGCACAGGCCTACTCTCTGCCCG TAGAAAAAGGCTGTTGCCCTTATAAAGCCGTTAATCAGTTCTACATGCTGGGGTCATATAGAGCCGATAA GGACGGGTTCCCGCTTGAAGAGTACTGGAAGGATCGCGAGGAATACCTGTTGAACCGGGAGCTGTGGGAA AAAAAGAATCAGCCCGGATATGATATCATCCAGGAAACTCTGGACTATTGTGCACTTGATGTTCTCGTTA CCGCAGAGCTGGTTACTAAACTGCAGGAATCCTATGCTCATTTCGTCAGGGACAGCGTGGGACTTCCACA CGCCCACTTTAACATTTTTCAGAGACCGACTATAAGCTCCAACTCACACGCCATTTTCAGGCAAATCGTA TATCGCACCGAGAAGCCTAATAGGACAAACCTTGGCCCCGGGCTGTTGGCCCCAAGCCACGAGCTCTATG ACTACGTTCGGGCTTCTATAAGAGGAGGGCGCTGTTACCCTACTTATATCGGAATCTTTGAGGACCCCAT TTATGTTTACGACATCTGCGGCATGTACGCCTCCGCCCTGACCCACCCAATGCCTTGGGGCAGCCCGCTG AATCCGTACGAAAGAGCCCTGGCCGCCCGAGAATGGCAGATGGCTTTGGACGATCCTACCCCTATCTCTT ATTTCGACAAAGATCTGCTGCCCGGCATTTTCACTATGGATGCAGATCCCCCCGATGAGTTGATGCTGGA CCCCCTCCCACCGTTTTGTTCACGCAAGGGGGGACGCCTTTGCTGGACAAACGAGCCCTTGCGCGGTGAA GTGGCAACATCCGTAGATCTGATTACGCTGCATAACCGAGGTTGGCAGGTACGCATTGTGCCAGACGAAC TCACAACAATTTTTCCCGAATGGAAATGCCTGGCCCGAGAGTACGTGCAGCTCAATATAGCAGCTAAAGA GAGAGCAGACAAAGAGAAAAATCAGACAATGCGGTCCATCGCCAAGCTCCTTAGTAACGCTCTGTATGGG AGCTTCGCCACAAAATTGGACAATAAAAAAATTGTATTTAGCGACCAGATGGATGAAAACCTTATGAAAG GCATCTCCACCGGTACTGTGAACATTAAGAGTAGCAGCTTCCTTGAGACCGATAACCTGAGCGCCGAAGT AATGCCCGCTTTCGAACGGGAATACCTGCCCCAGCAGCTGACCTTTTTGGACTCAGATCCAGAGGACTCA GAGGAAGAGCAGAGAACCGCACCGTTTTATACTCCGCCAGCCGGGACCCCAGGACACGTTAAGTATACGT ACAAGCCCATTACTTTCCTGGATGTTGAGGAGGGGGATATGTGTCTGCACACACTCGAAAAAGTGGATCC CCTGGTGGACAACGACAGATACCCAAGCCATATAGCAAGTTTTGTCCTGGCGTGGACAAGAGCTTTCGTG AGCGAGTGGTCTGAGTTTCTGTACGAAGAGGATAGAGGCACCCCGCTGGAGAAGCGGCCGGTGAAGTCAG TGTACGGCGATACGGATTCACTGTTCGTAACACAGCGGGGCCATGAGCTCATGGAAACAAAAGGCAAGAA GCGGATCAAAAAATACGGGGGGAAGCTGGTGTTCGATCCAAACGAACCTGACCTCACCTGGTTGGTGGAG TGCGAAACCGTCTGCGCCAGCTGCGGAGCCGACGCATACTCACCTGAGAGTATCTTCCTGGCTCCTAAAC TGTACGCCCTTAAATGTCTTTATTGCCCAGTTTGCAAAAAAACATCTAAAGGTAAGCTGCGGGCAAAGGG GCACGCTGCCGAGGCACTTAACTACGAGCTTATGGTGAATTGCTACCTGGCTGATATACAGGGGGCCAAA CGACAGAAGTTTTCTACCAGCAGAATGTCATTGAAGAGAACTCTCGCATCAGCCCAAGCCGGTGCACACC CCTTTACAGTGACCGAAACTACCCTTACTCGCACACTCAGACCATGGAAAGATCGGACGCTGGCCGCCCT TGACGCCCACCGACTGATCCCCTATTCCCGCTCCCGCCCTAATCCACGGAACGAGGAAGTGTGTTGGATC GAAATGCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100078 representing AP_000576 Red=Cloning sites Green=Tags MALVQAHGAGSFHPETSDTRRQSTRGGARKSSQSAIPEPARARRRRATTSHAPGSGTTAAARRASPSPLL SMQEAREPPPIKRKNKGTVVAPKGHGTLQAIDVSTNEPVEIKYHLNLPHALEKIMQVNLLSLPTNLSPQR LRTLDSSSLRALVLQLRPSRAEVWTCLPRGLVSMTTIETEDEQADTANIEEHEIQSPGLGFPLKFLVKGS QVQLIHEIQPVQRCDYCGRLYKHKHECSARRRNFYFHHINSQSSNWWQEIQFFPIGSHPRTERLFLTYDV ETYTWMGSFGKQLVPFMLVMKLSGEERLVNMAHDLAIKLKWDRWQQDPRTFYCVTPEKMAIGRHFRQYRD QLQTALAVDLWSSFLHANPHLHEWALEHYALSDPTDLTFDELSKLPHVRGTPRFIELYIVGHNINGFDEI VLAAQVINNRAEVPQPFRITRNFMPRAGKILFNDVTFALPNPAYKKRVDFQLWEQGACDDMDFKYQFLKV MVRDTFALTHASLRKAAQAYSLPVEKGCCPYKAVNQFYMLGSYRADKDGFPLEEYWKDREEYLLNRELWE KKNQPGYDIIQETLDYCALDVLVTAELVTKLQESYAHFVRDSVGLPHAHFNIFQRPTISSNSHAIFRQIV YRTEKPNRTNLGPGLLAPSHELYDYVRASIRGGRCYPTYIGIFEDPIYVYDICGMYASALTHPMPWGSPL NPYERALAAREWQMALDDPTPISYFDKDLLPGIFTMDADPPDELMLDPLPPFCSRKGGRLCWTNEPLRGE VATSVDLITLHNRGWQVRIVPDELTTIFPEWKCLAREYVQLNIAAKERADKEKNQTMRSIAKLLSNALYG SFATKLDNKKIVFSDQMDENLMKGISTGTVNIKSSSFLETDNLSAEVMPAFEREYLPQQLTFLDSDPEDS EEEQRTAPFYTPPAGTPGHVKYTYKPITFLDVEEGDMCLHTLEKVDPLVDNDRYPSHIASFVLAWTRAFV SEWSEFLYEEDRGTPLEKRPVKSVYGDTDSLFVTQRGHELMETKGKKRIKKYGGKLVFDPNEPDLTWLVE CETVCASCGADAYSPESIFLAPKLYALKCLYCPVCKKTSKGKLRAKGHAAEALNYELMVNCYLADIQGAK RQKFSTSRMSLKRTLASAQAGAHPFTVTETTLTRTLRPWKDRTLAALDAHRLIPYSRSRPNPRNEEVCWI EMP TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	AC_000019
ORF Size	3579 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AC_000019.1, AP_000576
RefSeq ORF	3579 bp
MW	136.3 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC100073	Myc-DDK-tagged ORF clone of viral ORF for E1A [Human adenovirus 35], codon optimized for human cell expression, AP_000571	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100074	Myc-DDK-tagged ORF clone of viral ORF for E1B 19K [Human adenovirus 35], codon optimized for human cell expression, AP_000572	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100075	Myc-DDK-tagged ORF clone of viral ORF for E1B 55K [Human adenovirus 35], codon optimized for human cell expression, AP_000573	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,990.00
VC100076	Myc-DDK-tagged ORF clone of viral ORF for IX [Human adenovirus 35], codon optimized for human cell expression, AP_000574	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100077	Myc-DDK-tagged ORF clone of viral ORF for IVa2 [Human adenovirus 35], codon optimized for human cell expression, AP_000575	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,510.00
VC100079	Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus 35], codon optimized for human cell expression, AP_000577	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,890.00
VC100080	Myc-DDK-tagged ORF clone of viral ORF for 52K [Human adenovirus 35], codon optimized for human cell expression, AP_000578	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,660.00
VC100081	Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus 35], codon optimized for human cell expression, AP_000579	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,030.00
VC100082	Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus 35], codon optimized for human cell expression, AP_000580	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,750.00
VC100083	Myc-DDK-tagged ORF clone of viral ORF for pVII [Human adenovirus 35], codon optimized for human cell expression, AP_000581	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100084	Myc-DDK-tagged ORF clone of viral ORF for V [Human adenovirus 35], codon optimized for human cell expression, AP_000582	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,180.00
VC100085	Myc-DDK-tagged ORF clone of viral ORF for pX [Human adenovirus 35], codon optimized for human cell expression, AP_000583	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100086	Myc-DDK-tagged ORF clone of viral ORF for pVI [Human adenovirus 35], codon optimized for human cell expression, AP_000584	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100087	Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus 35], codon optimized for human cell expression, AP_000585	10 ug 产品选项验证失败所有选项都是必需的	CNY 14,350.00
VC100088	Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus 35], codon optimized for human cell expression, AP_000586	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100089	Myc-DDK-tagged ORF clone of viral ORF for DBP [Human adenovirus 35], codon optimized for human cell expression, AP_000587	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,270.00
VC100090	Myc-DDK-tagged ORF clone of viral ORF for 100K [Human adenovirus 35], codon optimized for human cell expression, AP_000588	10 ug 产品选项验证失败所有选项都是必需的	CNY 12,350.00
VC100091	Myc-DDK-tagged ORF clone of viral ORF for 33K [Human adenovirus 35], codon optimized for human cell expression, AP_000589	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100092	Myc-DDK-tagged ORF clone of viral ORF for 22K [Human adenovirus 35], codon optimized for human cell expression, AP_000590	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100093	Myc-DDK-tagged ORF clone of viral ORF for pVIII [Human adenovirus 35], codon optimized for human cell expression, AP_000591	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100094	Myc-DDK-tagged ORF clone of viral ORF for E3 125K [Human adenovirus 35], codon optimized for human cell expression, AP_000592	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100095	Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-alpha0 [Human adenovirus 35], codon optimized for human cell expression, AP_000593	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100096	Myc-DDK-tagged ORF clone of viral ORF for E3 gp19K [Human adenovirus 35], codon optimized for human cell expression, AP_000594	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100097	Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-beta1 [Human adenovirus 35], codon optimized for human cell expression, AP_000595	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100098	Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-gamma1 [Human adenovirus 35], codon optimized for human cell expression, AP_000596	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100099	Myc-DDK-tagged ORF clone of viral ORF for E3 RID-alpha [Human adenovirus 35], codon optimized for human cell expression, AP_000597	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100100	Myc-DDK-tagged ORF clone of viral ORF for E3 RID-beta [Human adenovirus 35], codon optimized for human cell expression, AP_000598	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100101	Myc-DDK-tagged ORF clone of viral ORF for E3 147K [Human adenovirus 35], codon optimized for human cell expression, AP_000599	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100102	Myc-DDK-tagged ORF clone of viral ORF for U exon, partial [Human adenovirus 35], codon optimized for human cell expression, AP_000600	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100103	Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus 35], codon optimized for human cell expression, AP_000601	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100104	Myc-DDK-tagged ORF clone of viral ORF for E4 34K [Human adenovirus 35], codon optimized for human cell expression, AP_000603	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100105	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF6/7 [Human adenovirus 35], codon optimized for human cell expression, AP_000602	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100106	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF4 [Human adenovirus 35], codon optimized for human cell expression, AP_000604	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100107	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF3 [Human adenovirus 35], codon optimized for human cell expression, AP_000605	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100108	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF2 [Human adenovirus 35], codon optimized for human cell expression, AP_000606	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100109	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF1 [Human adenovirus 35], codon optimized for human cell expression, AP_000607	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00

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