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E1 (NC_001526) Virus Tagged ORF Clone
CAT＃: VC101899
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for E1 [Human papillomavirus type 16], codon optimized for human cell expression, NP_041327
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CNY 5,312.00
CNY 7,790.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101899 represents NCBI reference of NP_041327 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGATCCCGCAGGCACAAATGGGGAAGAGGGCACTGGATGTAATGGGTGGTTCTATGTCGAGGCCG TAGTGGAAAAAAAGACCGGCGATGCTATTTCAGACGACGAAAACGAGAATGACTCAGATACCGGCGAGGA CCTGGTCGATTTCATTGTGAACGACAACGATTACCTGACACAGGCTGAGACCGAGACAGCGCACGCTTTG TTCACCGCACAAGAGGCAAAACAGCATCGCGATGCCGTTCAGGTGCTGAAGCGAAAGTACCTTGTGAGCC CACTCAGCGATATCTCTGGCTGCGTGGATAATAATATCTCCCCGAGGCTGAAGGCAATTTGCATAGAGAA GCAGTCAAGGGCCGCGAAGCGACGGCTCTTTGAGAGCGAAGATTCCGGCTACGGCAACACCGAAGTCGAG ACCCAACAAATGCTGCAAGTCGAGGGACGACATGAGACCGAGACACCCTGTTCCCAGTACAGTGGAGGCT CAGGGGGCGGCTGCTCTCAATATTCTTCTGGATCAGGCGGGGAAGGGGTGTCAGAGAGGCACACAATCTG CCAAACCCCACTCACCAACATCCTGAATGTGCTCAAAACATCTAATGCAAAGGCGGCCATGCTGGCCAAG TTCAAGGAACTGTACGGAGTGTCATTTAGCGAGCTGGTGCGGCCCTTCAAGTCTAACAAAAGTACCTGTT GTGACTGGTGCATCGCTGCCTTTGGACTTACCCCCTCAATCGCGGACAGCATTAAAACACTCCTGCAGCA GTATTGCCTGTACTTGCATATCCAGTCATTGGCTTGTTCTTGGGGGATGGTTGTCCTGTTGCTTGTTCGG TACAAATGTGGCAAGAATCGCGAGACCATAGAGAAACTTCTGTCAAAGTTGCTGTGCGTGTCACCTATGT GCATGATGATCGAGCCCCCTAAGCTGCGAAGCACTGCAGCAGCCCTCTACTGGTACAAAACAGGTATCTC TAACATTAGTGAAGTCTATGGAGACACACCCGAGTGGATTCAGCGGCAAACTGTACTGCAGCATTCTTTC AATGACTGTACATTTGAACTCAGTCAAATGGTACAGTGGGCTTACGACAACGACATCGTTGATGATTCTG AAATAGCTTACAAATACGCCCAGCTGGCCGATACTAACAGCAACGCATCAGCTTTTTTGAAGAGCAACTC CCAAGCTAAGATTGTGAAGGACTGCGCCACTATGTGCAGGCACTATAAGCGCGCCGAAAAGAAACAAATG TCCATGTCTCAATGGATCAAATACAGATGTGATCGAGTCGACGATGGGGGCGACTGGAAACAGATCGTCA TGTTCCTGCGCTACCAGGGCGTCGAATTCATGTCCTTTCTCACCGCCCTCAAAAGATTTCTCCAGGGGAT CCCAAAGAAGAATTGTATTTTGTTGTATGGGGCCGCTAACACAGGCAAGTCCCTGTTCGGGATGTCCCTG ATGAAGTTTCTTCAAGGTAGTGTAATCTGCTTCGTGAACAGCAAATCCCACTTTTGGCTGCAGCCCCTTG CAGACGCTAAAATCGGTATGCTCGATGATGCAACAGTGCCATGTTGGAACTATATTGATGATAACTTGAG GAACGCACTCGATGGCAACCTGGTTTCCATGGACGTGAAGCACAGGCCGCTGGTTCAGCTGAAATGTCCG CCCCTGCTCATCACGAGCAATATCAATGCGGGAACTGATTCTCGGTGGCCTTACCTCCACAATCGGCTCG TGGTGTTCACATTCCCCAATGAGTTCCCCTTCGACGAAAATGGTAACCCTGTCTATGAACTGAATGACAA GAACTGGAAGTCCTTCTTTTCCCGCACCTGGAGCCGCCTGAGTCTGCACGAAGACGAGGATAAGGAGAAT GACGGCGACTCTCTGCCAACGTTTAAGTGCGTGTCTGGTCAAAACACTAACACCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101899 representing NP_041327
Red=Cloning sites Green=Tags MADPAGTNGEEGTGCNGWFYVEAVVEKKTGDAISDDENENDSDTGEDLVDFIVNDNDYLTQAETETAHAL FTAQEAKQHRDAVQVLKRKYLVSPLSDISGCVDNNISPRLKAICIEKQSRAAKRRLFESEDSGYGNTEVE TQQMLQVEGRHETETPCSQYSGGSGGGCSQYSSGSGGEGVSERHTICQTPLTNILNVLKTSNAKAAMLAK FKELYGVSFSELVRPFKSNKSTCCDWCIAAFGLTPSIADSIKTLLQQYCLYLHIQSLACSWGMVVLLLVR YKCGKNRETIEKLLSKLLCVSPMCMMIEPPKLRSTAAALYWYKTGISNISEVYGDTPEWIQRQTVLQHSF NDCTFELSQMVQWAYDNDIVDDSEIAYKYAQLADTNSNASAFLKSNSQAKIVKDCATMCRHYKRAEKKQM SMSQWIKYRCDRVDDGGDWKQIVMFLRYQGVEFMSFLTALKRFLQGIPKKNCILLYGAANTGKSLFGMSL MKFLQGSVICFVNSKSHFWLQPLADAKIGMLDDATVPCWNYIDDNLRNALDGNLVSMDVKHRPLVQLKCP PLLITSNINAGTDSRWPYLHNRLVVFTFPNEFPFDENGNPVYELNDKNWKSFFSRTWSRLSLHEDEDKEN DGDSLPTFKCVSGQNTNTL TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001526 |
| ORF Size | 1947 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_001526.2, NP_041327 |
| RefSeq ORF | 1947 bp |
| Locus ID | 1489075 |
| UniProt ID | P03120 |
| MW | 72.9 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101900 | Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV16gp5, partial [Human papillomavirus type 16], codon optimized for human cell expression, NP_041329 |
CNY 1,320.00 |
|
| VC101901 | Myc-DDK-tagged ORF clone of viral ORF for E5 protein [Human papillomavirus type 16], codon optimized for human cell expression, NP_041330 |
CNY 1,320.00 |
|
| VC101902 | Myc-DDK-tagged ORF clone of viral ORF for transforming protein [Human papillomavirus type 16], codon optimized for human cell expression, NP_041325 |
CNY 1,800.00 |
|
| VC101903 | Myc-DDK-tagged ORF clone of viral ORF for transforming protein [Human papillomavirus type 16], codon optimized for human cell expression, NP_041326 |
CNY 1,800.00 |
|
| VC101904 | Myc-DDK-tagged ORF clone of viral ORF for regulatory protein [Human papillomavirus type 16], codon optimized for human cell expression, NP_041328 |
CNY 5,488.00 |
|
| VC101905 | Myc-DDK-tagged ORF clone of viral ORF for minor capsid protein [Human papillomavirus type 16], codon optimized for human cell expression, NP_041331 |
CNY 5,488.00 |
|
| VC101906 | Myc-DDK-tagged ORF clone of viral ORF for major capsid L1 protein [Human papillomavirus type 16], codon optimized for human cell expression, NP_041332 |
CNY 5,928.00 |
