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E2 protein (NC_004102) Virus Tagged ORF Clone
CAT＃: VC102217
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751921
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CNY 3,656.00
CNY 4,370.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102217 represents NCBI reference of NP_751921 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCCATGTTACAGGGGGCAGCGCAGGCAGGACCACAGCTGGCCTCGTAGGGCTCCTGACCCCTG GTGCCAAGCAGAACATTCAGCTTATTAACACAAATGGCTCCTGGCATATAAACTCTACAGCACTGAATTG CAATGAGAGTCTTAATACGGGCTGGCTGGCAGGCCTCTTTTACCAACACAAGTTTAACTCTTCCGGATGT CCTGAAAGGTTGGCCAGCTGTCGCCGACTCACCGACTTTGCCCAGGGTTGGGGTCCTATCTCTTACGCCA ACGGGTCTGGACTCGATGAGCGCCCCTACTGCTGGCATTACCCGCCACGACCCTGCGGCATTGTGCCGGC AAAAAGTGTTTGCGGTCCCGTGTATTGCTTTACTCCATCCCCCGTGGTCGTCGGCACTACCGATCGGAGT GGCGCCCCTACATACTCATGGGGGGCTAACGACACCGACGTTTTTGTATTGAACAATACCAGGCCCCCTT TGGGCAACTGGTTTGGGTGTACTTGGATGAATTCTACAGGCTTCACGAAAGTGTGCGGAGCACCACCTTG CGTCATCGGGGGTGTGGGTAATAATACTCTGCTGTGCCCAACAGACTGTTTCCGGAAACACCCTGAGGCT ACGTATAGTAGGTGCGGCTCCGGCCCTTGGATAACACCTCGCTGCATGGTTGACTACCCCTATCGCCTTT GGCACTACCCATGTACAATCAACTATACCATTTTTAAGGTACGAATGTACGTCGGCGGCGTTGAACACCG GCTGGAGGCGGCATGTAACTGGACTAGGGGCGAACGCTGCGACCTGGAAGACAGAGACCGCTCCGAACTG TCTCCCCTGCTGCTGTCTACAACACAATGGCAAGTCTTGCCCTGCTCATTTACTACCCTGCCCGCACTGT CCACTGGGCTGATTCACCTGCACCAGAACATTGTGGATGTTCAGTACTTGTATGGCGTCGGCTCCTCTAT TGCCAGTTGGGCTATTAAGTGGGAATATGTAGTGCTCCTGTTCTTGCTCCTCGCAGACGCCCGAGTGTGC TCATGCCTGTGGATGATGCTGCTGATCTCCCAAGCCGAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102217 representing NP_751921
Red=Cloning sites Green=Tags METHVTGGSAGRTTAGLVGLLTPGAKQNIQLINTNGSWHINSTALNCNESLNTGWLAGLFYQHKFNSSGC PERLASCRRLTDFAQGWGPISYANGSGLDERPYCWHYPPRPCGIVPAKSVCGPVYCFTPSPVVVGTTDRS GAPTYSWGANDTDVFVLNNTRPPLGNWFGCTWMNSTGFTKVCGAPPCVIGGVGNNTLLCPTDCFRKHPEA TYSRCGSGPWITPRCMVDYPYRLWHYPCTINYTIFKVRMYVGGVEHRLEAACNWTRGERCDLEDRDRSEL SPLLLSTTQWQVLPCSFTTLPALSTGLIHLHQNIVDVQYLYGVGSSIASWAIKWEYVVLLFLLLADARVC SCLWMMLLISQAEA TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_004102 |
| ORF Size | 1092 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_004102.1, NP_751921 |
| RefSeq ORF | 1092 bp |
| MW | 40.2 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102214 | Myc-DDK-tagged ORF clone of viral ORF for protein F [Hepatitis C virus], codon optimized for human cell expression, NP_803170 |
CNY 1,200.00 |
|
| VC102215 | Myc-DDK-tagged ORF clone of viral ORF for core protein [Hepatitis C virus], codon optimized for human cell expression, NP_751919 |
CNY 3,600.00 |
|
| VC102216 | Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751920 |
CNY 2,400.00 |
|
| VC102218 | Myc-DDK-tagged ORF clone of viral ORF for p7 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751922 |
CNY 1,200.00 |
|
| VC102219 | Myc-DDK-tagged ORF clone of viral ORF for NS2 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751923 |
CNY 2,400.00 |
|
| VC102220 | Myc-DDK-tagged ORF clone of viral ORF for NS3 protease/helicase' [Hepatitis C virus], codon optimized for human cell expression, NP_803144 |
CNY 4,704.00 |
|
| VC102221 | Myc-DDK-tagged ORF clone of viral ORF for NS4A protein [Hepatitis C virus], codon optimized for human cell expression, NP_751925 |
CNY 1,200.00 |
|
| VC102222 | Myc-DDK-tagged ORF clone of viral ORF for NS4B protein [Hepatitis C virus], codon optimized for human cell expression, NP_751926 |
CNY 2,400.00 |
|
| VC102223 | Myc-DDK-tagged ORF clone of viral ORF for NS5A protein [Hepatitis C virus], codon optimized for human cell expression, NP_751927 |
CNY 3,656.00 |
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