BCMA (TNFRSF17) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA	Luciferase-Puro
Symbol	BCMA
Locus ID	608
Kit Components	KN208851G1, BCMA gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TAACGCTGACATGTTAGAGG KN208851G2, BCMA gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TTGCTTGAACGATTATTCAT KN208851LP-D, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette. Homologous arm and Luciferase-Puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet CCTTTTGCCT TTTGACTCCC AAAGTCCTGG GATTACAGGT GTGAACCCCT GCAGCTGGCC TCAATGTTAA GATCTTAAGG GGCACAGCAC AGACCTTGTC TTGTCTATCT CCCTGGCACC TCTCACAGAG TACAGCTTAT TAACAGATGT TCCAGAAACT GTTACTGAAC ATTCGGCTTG ATGCTGTGGG CTTGTCTGCA TCTTGCAACT GTCACCTGGC TGAGAAATTT CTTCTATAAA TAAGCAGTTT CTGTTTCAGA TGTGATATGC CCTGATATTT ACACCCTGTC TCTTACCCCA TCCAAGACTC AAACTTAGAA ACTTGAATTA GATGTGGTAT TCAAATCCTT AGCTGCCGCG AAGACACAGA CAGCCCCCGT AAGAACCCAC GAAGCAGGCG AAGTTCATTG TTCTCAACAT TCTAGCTGCT CTTGCTGCAT TTGCTCTGGA ATTCTTGTAG AGATATTACT TGTCCTTCCA GGCTGTTCTT TCTGTAGCTC CCTTGTTTTC TTTTTGTGAT CATGTTGCAG ATGGCTGGGC AGTGCTCCCA AAATGAATAT TTTGACAGTT TGTTGCATGC TTGCATACCT TGTCAACTTC ATATGGACTG CTTATTCAGA GAATCAACAT AATGGGCATG ATGGTGAGTT TTCTTGAATC AAAAAGAGAA AGGAAGCAAG GCAGTGATTT TAATGTTTAT GGAAACAAAG TAATTATTTG GAACTGAACT TGATATGATT CAGCACTATT AGCAACATAG ATTTTTTTTA AAAATCAGCT CTTCTAATTA AGTGATATTT AGAATTTAAA AGTCAATGTT CATTAATTAA GGTGATTGAA TGGAAATAAT CCATACTTGA TTATTTTGCT ATCAAAACAA TCCATAATTC ATTATTTTAG CAAAATAATC AAGTATGACA GCCGGGTGCG GTGGATGGCT CACACCTGTA ATCCCAGCAC TTTGGGAGGC CGAGGTGGGT GAATCACCTG AGGTCGGCAG TTTGAGGCCA GCCTGGCCAA CCTGGTGAAA CCCTGTCTCT ACTAAAAATA CAAAAAATTA GCTGGGCATG GTGGCACAGG TCTGTAATCC CAGCTACTCG GGAGGCTGAG GCAGGAGAAT TGTTTGAACT TGGGAGGTGG AGGTTGCAGT GAGCCGAGAT CGCGCCACTG CACTCTAGCC TGGGCAACAG AGCAAGACTT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001192
Synonyms	BCM; BCMA; CD269; TNFRSF13A
Summary	The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. [provided by RefSeq, Jul 2008]

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