THTPA (NM_024328) Human Mass Spec Standard

Specifications

Product Data
Description	THTPA MS Standard C13 and N15-labeled recombinant protein (NP_077304)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC200849
Predicted MW	25.6 kDa
Protein Sequence	Sequence Link (showhide) >RC200849 protein sequence Red=Cloning site Green=Tags(s) MAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDSGWELKCPGAA GVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVASFVTKRSAWKLVLLGADEEEP QLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSSMLGVPAQETAPAKLIVYLQRFRPQDYQRLL EVNSSRERPQETEDPDHCLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_077304
RefSeq Size	2228
RefSeq ORF	690
Synonyms	THTP; THTPASE
Locus ID	79178
Cytogenetics	14q11.2
Summary	This gene encodes an enzyme which catalyzes the biosynthesis of thiamine disphophate (vitamin B1) by hydrolysis of thiamine triphosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
Protein Pathways	Metabolic pathways, Thiamine metabolism

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