EAP30 (SNF8) (NM_007241) Human Mass Spec Standard

Specifications

Product Data
Description	SNF8 MS Standard C13 and N15-labeled recombinant protein (NP_009172)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC202137
Predicted MW	28.9 kDa
Protein Sequence	Sequence Link (showhide) >RC202137 protein sequence Red=Cloning site Green=Tags(s) MHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQFQD MCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLEELHQQVLKGRGKFAQDVS QDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTVVLQLAEKNGYVTVSEIKASLKWETERAR QVLEHLLKEGLAWLDLQAPGEAHYWLPALFTDLYSQEITAEEAREALP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_009172
RefSeq Size	1226
RefSeq ORF	774
Synonyms	Dot3; EAP30; VPS22
Locus ID	11267
Cytogenetics	17q21.32
Summary	The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
Protein Families	Transcription Factors
Protein Pathways	Endocytosis

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