OTUB1 (NM_017670) Human Mass Spec Standard

Specifications

Product Data
Description	OTUB1 MS Standard C13 and N15-labeled recombinant protein (NP_060140)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC210648
Predicted MW	31.3 kDa
Protein Sequence	Sequence Link (showhide) >Peptide sequence encoded by RC210648 Blue=ORF Red=Cloning site Green=Tag(s) MAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQ QKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDF HNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQE VEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYK TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC210648 also available, TP310648M
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_060140
RefSeq Size	2310
RefSeq ORF	813
Synonyms	HSPC263; OTB1; OTU1
Locus ID	55611
Cytogenetics	11q13.1
Summary	The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Protein Families	Protease

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