Trim32 (NM_001161782) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR209801 representing NM_001161782 Red=Cloning site Green=Tags(s) MAAAAAASHLNLDALREVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSINGVRCPFCSKITR ITSLTQLTDNLTVLKIIDTAGLSEAVGLLMCRGCGRRLPRQFCRSCGVVLCEPCREADHQPPGHCTLPVK EAAEERRRDFGEKLTRLRELTGELQRRKAALEGVSRDLQARYKAVLQEYGHEERRIQEELARSRKFFTGS LAEVEKSNSQVVEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVE LLKVGHVGPLQIGQAVKKPRTVNMEDSWAGEEGAASSASASVTFREMDMSPEEVAPSPRASPAKQRSSEA ASGIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQSEVLVADRGNYRIQVFNRKGFLKEIRRSPSGIDSFV LSFLGADLPNLTPLSVAMNCHGLIGVTDSYDNSLKVYTMDGHCVACHRSQLSKPWGITALPSGQFVVTDV EGGKLWCFTVDRGAGVVKYSCLCSAVRPKFVTCDAEGTVYFTQGLGLNVENRQNEHHLEGGFSIGSVGPD GQLGRQISHFFSENEDFRCIAGMCVDARGDLIVADSSRKEILHFPKGGGYSVLIREGLTCPVGIALTPKG QLLVLDCWDHCVKIYSYHLRRYSTP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	72.5 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_001155254
Locus ID	69807
UniProt ID	Q8CH72, Q3TLR3
Refseq Size	3193
Cytogenetics	4 34.43 cM
Refseq ORF	1965
Synonyms	3f3; 1810045E12Rik; BBS11; Zfp117
Summary	Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin). May ubiquitinate BBS2 (By similarity). Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha.[UniProtKB/Swiss-Prot Function]

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