Secondary Antibodies

Guinea Pig IgG (H&L) Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Biotin

F(Ab')2 Fragment Of Hamster IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Applications WB: 1:2,000-1:10,000
IHC: 1:300-1:2000
ELISA: 1:5000 - 1:25,000
Reactivities Hamster
Conjugation Biotin

Chicken IgM (mu chain) Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Biotin

Mouse IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
For histochemical and Cytochemical use are usually between 1/100 and 1/250
In ELISA and comparable non-precipitating antibody-binding assays between 1/1500 and 1/6000.
Reactivities Mouse
Conjugation Biotin

Turkey IgG (H+L chain) goat polyclonal antibody, Biotin

Applications Immunofluorescence.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry.
Reactivities Turkey
Conjugation Biotin

Mouse IgG (Fc specific) goat polyclonal antibody, Biotin

Applications ELISA: 1/20,000-1/100,000
Western blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:130,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Porcine IgG F(c) specific goat polyclonal antibody, Biotin

Applications ELISA: 1/10000-1/100000.
Western Blot: 1/10000-1/100000.
Reactivities Porcine
Conjugation Biotin

Porcine IgA goat polyclonal antibody, Biotin

Applications ELISA: 1/10000-1/100000.
Western Blot: 1/10000-1/100000
Reactivities Porcine
Conjugation Biotin

Human IgA (alpha chain) goat polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody
based assays using streptavidin or avidin conjugates:
ELISA: 1/60,000-1/600,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000
Reactivities Human
Conjugation Biotin

F(ab')2 Rat IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Biotin

Cat IgG (H&L) Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Biotin

Cat IgG F(c) Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Biotin

Bovine IgG F(ab')2 Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Biotin

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, Biotin, Aff - Purified

Applications Anti-Mouse IgG1 Biotin Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Biotin

Human IgA + IgG + IgM goat polyclonal antibody, Biotin

Applications Western Blot: 1/5000-1/20000.
ELISA: 1/400000.
Immunohistochemistry on frozen and paraffin sections: 1/1000-1/5000.
Reactivities Human
Conjugation Biotin