Monkey IgA (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/80, depending on the method used. |
| Reactivities | Monkey |
| Conjugation | FITC |

![Dot Blot showing the detection of Mouse IgG. A three-fold serial dilution of Mouse IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto (p/n [B501-0500]) 1 Hour at 20°C, Anti-Mouse IgG (H&L) (GOAT) Antibody Fluorescien Conjugated (p/n 610-102-121) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/610-102-121-mouse-igg-antibody-fluorescein-conjugated-pre-adsorbed-1-db-4x3.jpg?browse)

