Secondary Antibodies

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:800,000.
Western Blot: 1:5,000 - 1:50,000.
Immunohistochemistry: 1:1,000 - 1:5,000.
Reactivities Hamster
Conjugation Unconjugated

Hamster IgG (F(ab)2 specific) goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/100 - 1/200.
Reactivities Hamster
Conjugation FITC

Hamster IgG (F(ab)2 specific) goat polyclonal antibody, Purified

Applications ELISA: 1/1000-1/2000.
Reactivities Hamster, Reptiles
Conjugation Unconjugated

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/15,000-1/75,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Hamster
Conjugation HRP

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Hamster
Conjugation Texas Red

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Hamster
Conjugation TRITC

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Hamster
Conjugation FITC

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Hamster IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,500 of the reconstitution concentration is suggested for this product.
Reactivities Hamster
Conjugation AP

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, Biotin

Applications Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Western blot: 1:1,000 - 1:5,000.
ELISA: 1:10,000 - 1:50,000.
Immunohistochemistry.
Reactivities Hamster
Conjugation Biotin