Secondary Antibodies

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/25 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Flow cytometry (1/5 - 1/10): use 50 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Rat
Conjugation PE

Rat IgG2c goat polyclonal antibody, HRP

Applications ELISA: 1:10000.
Western Blot: 1:1000 - 1:10000.
Reactivities Rat
Conjugation HRP

Rat IgG1 goat polyclonal antibody, HRP

Applications ELISA: 1/10,000.
Western Blot: 1/1,000-1/10,000.
Reactivities Rat
Conjugation HRP

Rat IgM goat polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Western Blot: 1:1000 - 1:10,000.
Reactivities Rat
Conjugation HRP

Rat IgA goat polyclonal antibody, HRP

Applications ELISA: 1:10,000.
Western Blot: 1:1,000 - 10,000.
Reactivities Rat
Conjugation HRP

Rat IgG2a goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/500.
Reactivities Rat
Conjugation FITC

Rat IgG1 goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50 µl of 1/400 diluted antibody to label 1 x 106 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation TRITC

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Rat
Conjugation Unconjugated

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, FITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/2,000-1/10,000.
Immunofluorescence microscopy: 1/1,000-1/5,000.
Reactivities Rat
Conjugation FITC

Rat IgA (alpha chain) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation Texas Red

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilution:
This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Reactivities Rat
Conjugation AP

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation Texas Red

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1:20,000-1:100, 000.
Western blot: 1:2,000-1: 10,000.
Immunohistochemistry: 1:1,000-1:5,000.
Reactivities Rat
Conjugation Unconjugated