Secondary Antibodies

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Biotin

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), Biotin

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Immunoperoxidase staining of cytoplasmic IgA2(m)2, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/200 upwards.
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation Biotin

Canine IgE mouse monoclonal antibody, clone E6-71A1, Biotin

Applications ELISA: The antibody may be used as a detection antibody in ELISA techniques in combination with SM1498P as capture antibody.
Western Blotting: Under reducing conditions, this antibody detects a band of approximately 62-65 kDa in samples of affinity purified Dog IgE.
Reactivities Canine, Human
Conjugation Biotin

Rat IgE mouse monoclonal antibody, clone MARE-1, Biotin

Applications ELISA: 5 µg/ml as detecting antibody.
Western Blot.
Reactivities Rat
Conjugation Biotin

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, Biotin

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
ELISA: 1/5,000-1/20,000.
Reactivities Rat
Conjugation Biotin

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Biotin

Applications ELISA.
Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation Biotin

Rat IgM (Mu chain specific) mouse monoclonal antibody, Biotin

Applications Flow Cytometry (< / = 1.0 µg/10e6 cells).
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
ELISA.
Second step reagent for rat IgM Monoclonal Antibodies.
Surface IgM Detection.
B Cell Stimulation.
Reactivities Rat
Conjugation Biotin

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Biotin

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA: 1/5,000-1/10,000.
Reactivities Rat
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Biotin

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Reactivities Rat
Conjugation Biotin

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Biotin

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Biotin

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Biotin

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/100 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin

Goat IgG (H&L) Antibody Biotin Conjugated

Applications WB: 1:500 - 1:5,000
IHC: 1:500 - 1:5,000
ELISA: 1:5,000 - 1:50,000
Reactivities Goat
Conjugation Biotin

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, Biotin

Applications ELISA: 125ng/ml as detecting antibody.
Reactivities Rat
Conjugation Biotin