Secondary Antibodies

Human IgG (heavy chain) mouse monoclonal antibody, clone B33/20, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, Purified

Applications ELISA: 5 µg/ml as Coating antibody in combination with clone MARK-1/MARL-15 (AM05512HR-N) as Detection reagent and purified IgM (SA025).
Reactivities Rat
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone I27/mAb27

Applications FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2).
Usage recommendation
:
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Reactivities Human
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 4G12, Purified

Applications Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1 - 10 µg.
Reactivities Chicken
Conjugation Unconjugated

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Second step reagent for rat IgG1 Monoclonal Antibodies.
Reactivities Rat
Conjugation Unconjugated

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-4, Purified

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Indirect Immunofluorescence staining. (Ref.1)
In ovo suppression of Chicken B lymphocytes.
In vitro activation of Chicken peripheral B lymphocytes.
Reactivities Chicken
Conjugation Unconjugated

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, Purified

Applications Flow Cytometry and Immunofluorescence Microscopy:
Identification and enumeration of IgM+ cells.
ELISA.
Second step reagent for rat IgM Monoclonal Antibodies.
Surface IgM Detection.
B Cell Stimulation.
Reactivities Rat
Conjugation Unconjugated

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Second step reagent for rat IgG2c Monoclonal Antibodies.
Reactivities Rat
Conjugation Unconjugated

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
ELISA.
Second step reagent for rat IgG2a Monoclonal Antibodies.
Reactivities Rat
Conjugation Unconjugated

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
ELISA.
Second step reagent for rat IgG2b Monoclonal Antibodies.
Reactivities Rat
Conjugation Unconjugated

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA.
Second step reagent for rat IgG1 Monoclonal Antibodies.
Reactivities Rat
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified

Applications Immunoassay.
Western Blot (non-recduced).
The typical starting working dilution is 1/10.
Immunoprecipitation.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified

Applications Immunoassays.
Western blot (non-reduced): The typical starting working dilution is 1:10.
Also useful for immunopurification.
Reactivities Chicken, Duck
Conjugation Unconjugated

IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated