Secondary Antibodies

Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), Purified

Applications ELISA.
Western Blot.
Immunoblotting.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation Unconjugated

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, Purified

Applications ELISA.
Western Blot.
Immunoblotting.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 4H10, Purified

Applications Flow Cytometry: 4 µg/ml.
ELISA.
Western Blot.
Reactivities Human
Conjugation Unconjugated

Goat IgG (H+L chain) mouse polyclonal antibody, Serum

Applications ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunochemistry: 1/1,000-1/5,000.
Reactivities Goat
Conjugation Unconjugated

Goat IgG (H&L) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Goat
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone I27/mAb27

Applications FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2).
Usage recommendation
:
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Reactivities Human
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 4G12, Purified

Applications Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1 - 10 µg.
Reactivities Chicken
Conjugation Unconjugated

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-8, Purified

Applications ELISA: 5 µg/ml (as a coating antibody).
Reactivities Rat
Conjugation Unconjugated

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-3, Purified

Applications ELISA: 5ug/ml; This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgG2b (SA023).
Reactivities Rat
Conjugation Unconjugated

Rat IgG2a (heavy chain) mouse monoclonal antibody, clone MARG2a-1, Purified

Applications ELISA: 1.25 µg/ml; This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgA (SA021).
Reactivities Rat
Conjugation Unconjugated

Rat IgG1 (heavy chain) mouse monoclonal antibody, clone MARG1.2, Purified

Applications ELISA: 3-5 µg/ml.
This antibody may be used as a Coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified Rat IgG1 (Cat.-No SA013).
Reactivities Rat
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone R1/69, Supernatant

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature).
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain, Fc-Region) (in vitro) mouse monoclonal antibody, clone 2B9

Reactivities Human
Conjugation Unconjugated

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, Purified

Applications ELISA: ≤ 5 µg/ml (Ref.1)
Immunofluorescence: ≤ 1 µg/10e6 cells.
Flow Cytometry.
Reactivities Human
Conjugation Unconjugated

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-1, Purified

Applications Flow Cytometry.
Immunoprecipitation. (Ref.1)
Reactivities Chicken
Conjugation Unconjugated