Secondary Antibodies

Human IgM mouse monoclonal antibody, clone R1/69, Supernatant

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature).
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain, Fc-Region) (in vitro) mouse monoclonal antibody, clone 2B9

Reactivities Human
Conjugation Unconjugated

Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, FITC

Applications Direct Immunofluorescence analysis: 2-5 µg/ml.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone 4H10, FITC

Applications Flow cytometry: 2-5 µg/ml.
Reactivities Human
Conjugation FITC

Human IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:500-1:2,500
Reactivities Human
Conjugation TRITC

Kappa light chain, mouse anti human, clone κ -117 Biotin

Applications Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.25µg/ml (1:800) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Biotin

Lambda light chain, mouse anti human, clone λ-1 Biotin

Applications Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.125µg/ml (1:1600) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Biotin

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, Purified

Applications ELISA: ≤ 5 µg/ml (Ref.1)
Immunofluorescence: ≤ 1 µg/10e6 cells.
Flow Cytometry.
Reactivities Human
Conjugation Unconjugated

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, PE

Applications ELISA.
Immunofluorescence < / = 0.2 µg/10e6 cells.
Reactivities Human
Conjugation PE

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, Biotin

Applications ELISA: 1/5,000-1/20,000.
Immunofluorescence < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation Biotin

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, Biotin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA: 1/5,000-1/10,000.
Flow Cytometry: 1 µg / 10e6 cells
Western Blotting
Reactivities Human
Conjugation Biotin

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, Biotin

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA: 1/5,000-1/10,000.
Reactivities Human
Conjugation Biotin

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Biotin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA: 1/10,000-1/20,000.
Reactivities Human
Conjugation Biotin

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, Biotin

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA: 1/5,000-1/10,000.
Reactivities Human
Conjugation Biotin

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, Biotin

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/10e6 cells.
ELISA: 1/1,000-1/2,000.
Reactivities Human
Conjugation Biotin