Secondary Antibodies

Canine IgE mouse monoclonal antibody, clone E6-71A1, Purified

Applications ELISA.
Western Blotting: Under reducing conditions, this antibody detects a single major band of approximately 62-65 kDa in samples of affinity purified Dog IgE.
Reactivities Canine
Conjugation Unconjugated

Rat IgD (heavy chain) mouse monoclonal antibody, clone MARD-3, Purified

Applications Immunohistochemistry on Frozen Sections.
ELISA: This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent.
Reactivities Rat
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Low Endotoxin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Purified

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, Purified

Applications Fluorescent-linked-immunosorbent assay (FLISA)
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry (frozen Sections).
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, Purified

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA. 1 ug/mL
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, Low Endotoxin

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, Purified

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Purified

Applications ELISA.
Flow Cytometry.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY (gamma chain specific) mouse monoclonal antibody, clone G-1, Purified

Applications ELISA.
Flow Cytometry.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Chicken
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone NI 179, Purified

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, immunoblotting, immunoprecipitation, indirect immunoperoxidase and immunofluorescence
General Recommended Dilutions:
Histochemical use: 1/50-1/200
Dilutions may be prepared by adding PBS, pH 7.2.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone NI 179, Biotin

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgM, and Immunoblotting. As a second step an Avidin or Streptavidin conjugate have to be used.
General Recommended Dilutions:
Histochemical Use: 1/100-1/500
ELISA: from 1/1,000 upwards
Western blot: from 1/2,000 upwards
Reactivities Human
Conjugation Biotin

Human IgM mouse monoclonal antibody, clone NI 179, FITC

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
Reactivities Human
Conjugation FITC

Human IgM mouse monoclonal antibody, clone NI 179, Ascites

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgM, and Immunoblotting. The optimum working
dilution is an assay-related characteristic and should always be determined by titration.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards
Western blot: from 1/1,000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, HRP

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/500
ELISA: from 1/1000 upwards
Western blot: from 1/2000 upwards.
Reactivities Human
Conjugation HRP