Biotin Conjugated Rabbit Anti-human IgG
| Applications | WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000 |
| Reactivities | Human IgG |
| Conjugation | Biotin |
Secondary Antibodies
Mouse IgE (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/3000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Monkey IgG (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: Histochemical and cytochemical: 1/100 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/2000 - 1/10000. |
| Reactivities | Monkey |
| Conjugation | Biotin |
Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | Can be usde in immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and cytochemical: 1/100 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/2000 - 1/20000. |
| Reactivities | Monkey |
| Conjugation | Biotin |
Monkey IgG (Fab specific) rabbit polyclonal antibody, Biotin
| Applications | In immunocytochemical and immunohistochemical staining to identify and measure free or Ig bound subunits of both kappa and lambda type at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. |
| Reactivities | Monkey |
| Conjugation | Biotin |
Bovine IgM (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Recommneded Dilutions: Histochemistry and Cytochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/100-1/500. |
| Reactivities | Bovine |
| Conjugation | Biotin |
Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and cytochemical Use: 1/100- 1/2500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Biotin
| Applications | ELISA: 1/1000 - 1/2000. Flow Cytometry: 1/50 - 1/100; Use 50µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on frozen and paraffin embedded sections: 1/50 - 1/200. |
| Reactivities | Mouse |
| Conjugation | Biotin |
CNY 5742.00
4周
Biotin Conjugated Rabbit Monoclonal Anti-Human Ig Light Chains antibody 3, clone RM129
| Applications | ELISA: 0.02ug/mL – 0.25ug/mL; IHC, ICC: 0.5ug/mL – 2ug/mL |
| Reactivities | Human Ig light chain |
| Conjugation | Biotin |
F(ab')2 Dog IgG (H&L) Antibody Biotin Conjugated
| Applications | WB: 1:2,000-1:10,000 IHC: 1:1,000-1:5,000 ELISA: 1:20,000 - 1:100,000 |
| Conjugation | Biotin |
Sheep IgG (H&L) Secondary Antibody Biotin Conjugated Pre-Adsorbed
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:20,000 - 1:100,000 |
| Conjugation | Biotin |
Mouse IgG (H&L) Secondary Antibody Biotin Conjugated
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:200,000 - 1:300,000 |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgG (H&L) Secondary Antibody Biotin Conjugated
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:300,000 |
| Reactivities | Mouse |
| Conjugation | Biotin |
Porcine IgG (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of swine origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. |
| Reactivities | Porcine |
| Conjugation | Biotin |
Sheep IgM (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000. |
| Reactivities | Sheep |
| Conjugation | Biotin |
