Secondary Antibodies

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
Western blot: 1/1,000-1/10,000.
ELISA: 1/10,000-1/50,000.
Immunohistochemistry: 1/500-1/2500.
Reactivities Chicken
Conjugation HRP

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/10,000.
Immunohistochemistry: 1/500-2,500.
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation HRP

Rat IgG (H+L chain), Fab Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/1000 - 1/2000.
Immunohistochemistry on frozen sections: 1/50.
Immunohistochemistry on paraffin sections: 1/50.
Immunohistochemistry on resin sections: 1/50.
Reactivities Rat
Conjugation HRP

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/500 - 1/1000.
Western Blot: 1/2000 - 1/5000.
Immunohistochemistry on frozen, paraffin and resin sections: 1/50.
Reactivities Mouse
Conjugation HRP

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions
ELISA: 1/10,000-1/50,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Goat
Conjugation HRP

Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Reactivities Goat
Conjugation HRP

Goat IgM rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Goat
Conjugation HRP

Goat IgA rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Goat
Conjugation HRP

Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reactivities Chicken
Conjugation HRP

Chicken IgG / Chicken IgY (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:37,000 of the reconstitution concentration is suggested for this product.
Reactivities Chicken
Conjugation HRP

Donkey IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Note: This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product.
Reactivities Donkey
Conjugation HRP

Goat IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Western Blot/Dot Blot: 1/1,000-1/10,000 or Dot blot.
Immunohistochemistry: 1/500-1/2,500.
ELISA: This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 is suggested for this product.
Reactivities Goat
Conjugation HRP

Goat IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3- thylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:62,000 of the reconstitution concentration is suggested for this product.
Reactivities Goat
Conjugation HRP

Canine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for this product.
Reactivities Dog
Conjugation HRP

Feline IgG F(ab')2 specific rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:30,000 of the reconstitution concentration is suggested for this product.
Reactivities Feline
Conjugation HRP