Secondary Antibodies

Goat IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA: 1/50,000-1/100,000. 
Western Blot: 1/5,000-1/20,000. 
Immunohistochemistry: 1/1,000-1/5,000. 
Note: HRP Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Reactivities Goat
Conjugation HRP

Anti-Goat IgG [H&L] HRP Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:20,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:120,000
Reactivities Goat
Conjugation HRP

Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Reactivities Goat
Conjugation HRP

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions
ELISA: 1/10,000-1/50,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Goat
Conjugation HRP

Goat IgM rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Goat
Conjugation HRP

Goat IgA rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Goat
Conjugation HRP

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product.
Reactivities Goat
Conjugation HRP

Goat IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:35,000 of the reconstitution concentration is suggested for this product.
Reactivities Goat
Conjugation HRP

Goat IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Western Blot/Dot Blot: 1/1,000-1/10,000 or Dot blot.
Immunohistochemistry: 1/500-1/2,500.
ELISA: This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 is suggested for this product.
Reactivities Goat
Conjugation HRP

Goat IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3- thylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:62,000 of the reconstitution concentration is suggested for this product.
Reactivities Goat
Conjugation HRP