Human IgG rabbit polyclonal antibody, Ig Fraction
| Applications | Immunoprecipitation. Immunodiffusion. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Secondary Antibodies
Human IgG rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/200-1/500 dilution in an ABC method (30 minutes at room temperature). Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Recommended Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/200-1/500 dilution in an ABC method (30 minutes at room temperature). Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Recommended Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended dilutions: ELISA: 1:10,000-1:50,000. Western Blot: 1:1,000-1:5,000. Immunohistochemistry: 1:500-1:3,000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/350,000. Western Blot: 1/3,000-1/15,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (gamma chain specific) rabbit polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Reactivities | Human |
| Conjugation | FITC |
Rat IgG2c rabbit polyclonal antibody, Purified
| Applications | ELISA: 1µg/ml - 10µg/ml. Western Blot. Flow Cytometry. |
| Reactivities | Human, Rat |
| Conjugation | Unconjugated |
Human IgG (H&L) Antibody
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:20,000 - 1:100,000 |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/300,000 Western Blot: 1/5,000-1/25,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgM rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes. Positive control: Tonsil. Cellular localization: Cytoplasmic. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes. Positive control: Tonsil. Cellular localization: Cytoplasmic. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/10,000. Immunohistochemistry: 1/500-2,500. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgG (gamma chain specific) rabbit polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:35,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Human |
| Conjugation | Biotin |
Human IgG rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Immunodiffusion. For Ouchterlony: Sample Size: 15 µl Distance between wells: 5 mm Test time: 4 days at 37°C Antigens tested: Normal human serum, purified human IgG, normal mouse serum. Results: Titre with NHS > 1:1000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (Fc specific) rabbit polyclonal antibody, Texas Red
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Reactivities | Human |
| Conjugation | Texas Red |
