Secondary Antibodies

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Mouse IgM (Fc specific) Rabbit Polyclonal Antibody
AP32992FC-N

CNY 7710.00

5周

Mouse IgM (Fc specific) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
(In)direct immunofluorescence.
In direct staining of cytoplasmic IgM in fixed mouse cells and tissue substrates; to identify circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20- 1/80.
Reactivities Mouse
Conjugation FITC
Mouse IgM (Fc specific) Rabbit Polyclonal Antibody
AP32992AF-N

CNY 6580.00

5周

Mouse IgM (Fc specific) rabbit polyclonal antibody, Azide Free

Applications Can be used:
As unlabelled primary or secondary antibody reagent for indirect detection of IgM in Mouse cells, tissues and body fluids in Immunofluorescence and Immunoenzyme assay methods.
For the production of immunoconjugates with a selected marker.
To prepare immunoaffinity adsorbents by coupling to an artificial carrier.
In non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent.
In Western blotting.
When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling techniques, the optimum concentration of this product should be established before.
Recommneded Working Dilutions:
Histochemistry: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Precipitin titre: not less than 1/64 when tested against normal Mouse serum in agar-block immunodiffusion titration.

Performance testing: Titre, specificity and reactivity of the subclass specific cross-adsorbed IgG is further evaluated in a number of highly sensitive non-precipitating antibody-binding assay systems. These performance tests include direct single and double identification of cIg in mouse cell and tissue substrates and their evaluation as second antibody in the analysis of human cells and tissues after reacting with a primary monoclonal mouse antibody to a human antigen. The quantitative specific recognition ability is verified in double staining procedures together with reference reagents of known specificity and reactivity.
Reactivities Mouse
Conjugation Unconjugated
Mouse IgG (Fc specific) Rabbit Polyclonal Antibody
R1352P

CNY 2657.00

4周

Mouse IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications Immunoprecipitation.
Immunodiffusion.
Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1/40,000-1/180,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated
Mouse IgG (H+L chain), F(ab)2 Fragment Rabbit Polyclonal Antibody
SP1012B

CNY 11960.00

4周

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Biotin

Applications ELISA: 1/1000 - 1/2000.
Flow Cytometry: 1/50 - 1/100; Use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunohistochemistry on frozen and paraffin embedded sections: 1/50 - 1/200.
Reactivities Mouse
Conjugation Biotin
Mouse IgG (H&L) Secondary Antibody Biotin Conjugated
TA397920

CNY 1744.00

4周

Mouse IgG (H&L) Secondary Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:200,000 - 1:300,000
Reactivities Mouse
Conjugation Biotin
Mouse IgG (H&L) Secondary Antibody Biotin Conjugated
TA397921

CNY 1219.00

4周

Mouse IgG (H&L) Secondary Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:300,000
Reactivities Mouse
Conjugation Biotin
Mouse IgM (mu chain) Antibody Peroxidase Conjugated
TA397912

CNY 4200.00

4周

Mouse IgM (mu chain) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:180,000
Reactivities Mouse
Conjugation HRP
Mouse IgM (H&L) Antibody Peroxidase Conjugated
TA397267

CNY 9038.00

4周

Mouse IgM (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation HRP
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
R1253B

CNY 2877.00

4周

Mouse IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Biotin
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
R1253AP

CNY 3224.00

4周

Mouse IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for use in Immunoblotting (Western or dot blot), ELISA, Immunohistochemistry and other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000-1/10,000.
Western blot: 1/500-1/2,500.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Mouse
Conjugation AP
Mouse IgG1 (gamma 1 chain specific) Rabbit Polyclonal Antibody
R1359HRP

CNY 4369.00

4周

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, HRP

Applications Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western blotting as well as other anti IgG1 antibody based assays.
Recommended Dilutions:
ELISA: 1/300,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP
Mouse IgG2a (gamma 2a chain specific) Rabbit Polyclonal Antibody
R1360HRP

CNY 4369.00

4周

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, HRP

Applications This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, Western-blot, Immunoprecipitation and more generally immunoassays.
Anti-Mouse IgG2a heavy chain antibody is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/90,000
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP
Mouse IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody
R1350P

CNY 2963.00

4周

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA : 1:20,000 - 1:40,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Reactivities Mouse
Conjugation Unconjugated
Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Biotin Conjugated
TA397908

CNY 3713.00

4周

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation Biotin
Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Peroxidase Conjugated
TA397907

CNY 3713.00

4周

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Peroxidase Conjugated

Applications WB: 1:2000 - 1:10000
IHC: 1:1000 - 1:5000
ELISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation HRP