Human IgA (Fc specific) sheep polyclonal antibody, TRITC
| Applications | Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgA. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. Recommended Dilutions: 1/10-1/40 This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Human |
| Conjugation | TRITC |


![Western Blot of Anti-Rat IgG (H&L) (SHEEP) Antibody (p/n 612-6102). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n [D310-05]). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (SHEEP) Antibody (p/n 612-6102) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Sheep IgG (DONKEY) Peroxidase Conjugated Antibody (p/n 613-703-168) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/612-6102-anti-rat-igg-sheep-antibody-1-wb-4x3.jpg?browse)