Secondary Antibodies

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC

Mouse IgA goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Immunofluorescence: neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse, Rat
Conjugation FITC

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, FITC

Applications FLISA: 1/200-1/400.
Flow Cytometry:
  1 ≤ µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, FITC

Applications Flow Cytometry: < / = 1.0 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA:  1:10,000/1:50,000
IF Microscopy:  1:1000/1:5000
Reactivities Rat
Conjugation FITC

Rat IgG2a goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/500.
Reactivities Rat
Conjugation FITC

Rat IgG1 goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50 µl of 1/400 diluted antibody to label 1 x 106 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/25 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, FITC

Applications Flow Cytometry: 10 µg/ml; use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgG2c (heavy chain) mouse monoclonal antibody, clone MARG2c-5, FITC

Applications Flow cytometry: 5 µg/ml; use 10 µl of diluted antibody to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, FITC

Applications Flow cytometry (5 µg/ml): use 50 µl of the suggested working dilution to label
10e6 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgE mouse monoclonal antibody, clone MARE-1, FITC

Applications Flow Cytometry: Use 50 µl of 5 µg/ml diluted antibody to label 2 x 10e7 cells in 100 µl.
Reactivities Rat
Conjugation FITC