Secondary Antibodies

Human IgE mouse monoclonal antibody, clone MH25/1, Purified

Applications Immunofluorescence.
Immunohistochemistry on frozen and paraffin sections: 1/5 – 1/10 (Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but the optimal dilution should be tested by serial dilution.) 
Dilute immediately before use with PBS or TRIS.
Localisation: D ε 1 epitope on Fc Fragment 
Positive control: Polypus nose
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone MK1A6, Purified

Applications ELISA assay development of total IgG.
Passive hemagglutination assays.
Histological staining of Acetone Fixed Frozen tissue sections.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone R1/69, Supernatant

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature).
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgM rabbit polyclonal antibody, Purified

Applications Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes.
Positive control: Tonsil.
Cellular localization: Cytoplasmic.
Reactivities Human
Conjugation Unconjugated

Human IgM rabbit polyclonal antibody, Purified

Applications Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes.
Positive control: Tonsil.
Cellular localization: Cytoplasmic.
Reactivities Human
Conjugation Unconjugated

Human IgA mouse monoclonal antibody, clone DA43D3, Purified

Applications Suitable for Immunohistochemistry on Frozen (1 µg/ml, 1/400) and Paraffin Embedded Sections. Proteinase K pretreatment for antigen retrieval is recommended.
Suggested Positive Control: Human tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone DM3A3, Purified

Applications Immunohistochemistry on frozen sections: 1-2 µg/ml (1:200 - 1:400).
Immunohistochemistry on paraffin sections: Proteinase K pretreatment for antigen retrieval is recommended.
Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Unconjugated