Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP
| Applications | Sandwich ELISA: 1/120,000 dilution of antibody was found to generate an O.D of 1.0 in a 15 minute reaction with Tetramethyl Benzidine as the substrate. Western blot (1/5000). Immunoelectrophoresis. |
| Reactivities | Chicken |
| Conjugation | HRP |
Chicken IgM (Fc specific) goat polyclonal antibody, HRP
| Applications | Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in chicken serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. |
| Reactivities | Chicken |
| Conjugation | HRP |
Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 9B4, Purified
| Applications | Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml. Immunoprecipitation: 1-10 µg. |
| Reactivities | Chicken |
| Conjugation | Unconjugated |
Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 08C, Purified
| Applications | ELISA. Immunoblotting. |
| Reactivities | Chicken |
| Conjugation | Unconjugated |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Aff - Purified
| Applications | ELISA: 1/10,000 dilutions produced a detectable signal, as measured using HRP-labeled Chicken anti-Goat IgG Antibody Cat.-No AP31796HR-N (1/5000 dilution). Western blot (1/5000). Immunocytochemistry. Immunohistochemistry (1/250). Immunoprecipitation (1/250). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and A30-106-10 (75 μl) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough. |
| Reactivities | Chicken |
| Conjugation | Unconjugated |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin
| Applications | ELISA. Western Blot (1/5000). Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA using HRP-labeled streptavidin. A 1/500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with Tetramethylbenzidine as the substrate. |
| Reactivities | Chicken |
| Conjugation | Biotin |
Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 4G12, Purified
| Applications | Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml. Immunoprecipitation: 1 - 10 µg. |
| Reactivities | Chicken |
| Conjugation | Unconjugated |
Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Biotin
| Reactivities | Chicken |
| Conjugation | Biotin |
Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified
| Applications | Immunoassay. Western Blot (non-recduced). The typical starting working dilution is 1/10. Immunoprecipitation. |
| Reactivities | Chicken |
| Conjugation | Unconjugated |
Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified
| Applications | Immunoassays. Western blot (non-reduced): The typical starting working dilution is 1:10. Also useful for immunopurification. |
| Reactivities | Chicken, Duck |
| Conjugation | Unconjugated |
Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Biotin
| Applications | Immunoassays. Western blot (non-reduced): The typical starting working dilution is 1/10. |
| Reactivities | Chicken, Duck |
| Conjugation | Biotin |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP
| Applications | ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate. Western Blot (1/1000). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough. Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA. |
| Reactivities | Chicken |
| Conjugation | AP |
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