Secondary Antibodies
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining to identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000 - 1/20000. |
| Reactivities | Rabbit |
| Conjugation | Biotin |
CNY 4026.00
4周
Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), AP, Purified
| Applications | ELISA: 1/1000-1/2000 (See also Ref.2-9). FLISA. Applications Reported in Literature: Flow Cytometry (Ref.15,18,19). Immunohistochemistry on Frozen Sections (Ref.10). Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11). Immunocytochemistry (Ref.12). Immunoblotting (Ref.3,13-16). Microarray (Ref.17). Multiplex (Ref.2,20-22). Purification (Ref.15). |
| Reactivities | Human |
| Conjugation | AP |
Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, Purified
| Applications | ELISA: 1/4,000-1/8,000. Flow Cytometry. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, Purified
| Applications | ELISA: 5 µg/ml. This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent and purified Rat IgA (Cat.-No SA013) as antigen. Immunofluorescence. Immunohistochemistry on Frozen Sections. |
| Reactivities | Rat |
| Conjugation | Unconjugated |
Cy3 Conjugated Rabbit Anti-Goat IgG
| Applications | IF/ICC: 1:50-100; IHC: 1:50-100 |
| Reactivities | Goat IgG |
| Conjugation | Cy3 |
Mouse IgE (Fc specific) goat polyclonal antibody, TRITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/20 - 1/60, depending on the method used. |
| Reactivities | Mouse |
| Conjugation | TRITC |
CNY 6190.00
5周
Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Ascites
| Applications | To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Rabbit Monoclonal Anti-Mouse Kappa Light Chain antibody, clone RM103
| Applications | ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB (non-reduced): 0.1ug/mL-0.5ug/mL |
| Reactivities | Mouse Ig kappa light chain |
| Conjugation | Unconjugated |
Mouse IgG (Fc specific) goat polyclonal antibody, HRP
| Applications | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000 Western blot: 1/1,000-1/5,000 Flow Cytometry: 1/500-1/2,500 |
| Reactivities | Mouse |
| Conjugation | HRP |
Canine IgG2 Sheep polyclonal antibody, Purified
| Applications | ELISA: 1:100 - 1:10,000. Western Blot: 1:1000 - 1:10,000. Immunohistochemistry on frozen sections: 1:200 - 1:2000. |
| Reactivities | Canine |
| Conjugation | Unconjugated |
Rabbit IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in rabbit serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000 - 1/10000. |
| Reactivities | Rabbit |
| Conjugation | Biotin |
Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and cytochemical Use: 1/100- 1/2500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Rabbit Monoclonal Anti-Mouse IgG (gamma-Chain) antibody, clone RM104
| Applications | ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB (non-reduced): 0.1ug/mL-0.5ug/mL |
| Reactivities | Mouse IgG gamma |
| Conjugation | Unconjugated |
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Reactivities
- Mouse (49)
- Human (38)
- Rabbit (23)
- Rat (13)
- Goat (8)
- Monkey (4)
- Canine (2)
- Chicken (2)
- Mouse IgG (2)
- Porcine (2)
- Bovine (1)
- Feline (1)
- Goat IgG (1)
- Hamster (1)
- Human Ig light chain (1)
- Human IgA1 (1)
- Human IgG (1)
- Human IgG1 (1)
- Mouse Ig kappa light chain (1)
- Mouse IgG gamma (1)
- Mouse IgG1 (1)
- Mouse IgM mu-chain (1)
- Pig (1)
- Rabbit IgG (1)
- Rabbit IgG Fab (1)
- Sheep (1)
