Secondary Antibodies

Biotin Conjugated Rabbit Monoclonal Anti-Human Ig Light Chains antibody 3, clone RM129

Applications ELISA: 0.02ug/mL – 0.25ug/mL; IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human Ig light chain
Conjugation Biotin

Rabbit Monoclonal Anti-Human IgA1 antibody, clone RM124

Applications ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human IgA1
Conjugation Unconjugated

Human IgG rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/200-1/500 dilution in an ABC method (30 minutes at room temperature).
Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Rat IgG2a goat polyclonal antibody, HRP

Applications ELISA: 1/10,000.
Western Blot: 1/1,000-1/10,000.
Reactivities Rat
Conjugation HRP

Human IgG mouse monoclonal antibody, clone B573M, Purified

Applications ELISA.
Lateral Flow Assays.
Reactivities Human
Conjugation Unconjugated

Porcine IgA goat polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Porcine
Conjugation HRP

Human IgG (F(ab)2 specific) goat polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/20-1/80.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation FITC

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), Ascites

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Ascites

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Rabbit IgG (H+L chain) goat polyclonal antibody, Texas Red, Lyophilized

Applications Suitable for immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
IF Microscopy: 1/1000 - 1/5000.
Reactivities Rabbit
Conjugation Texas Red

Mouse IgG (Fc specific) goat polyclonal antibody, Biotin

Applications ELISA: 1/20,000-1/100,000
Western blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:130,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Rat IgE mouse monoclonal antibody, clone MARE-1, Biotin

Applications ELISA: 5 µg/ml as detecting antibody.
Western Blot.
Reactivities Rat
Conjugation Biotin

Rat IgE mouse monoclonal antibody, clone MARE-1, HRP

Applications ELISA: 0.5 µg/ml.
Western Blot.
Reactivities Rat
Conjugation HRP

Human IgG mouse monoclonal antibody, clone 4D2D9G8, Purified

Applications Western Blot: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Reactivities Human
Conjugation Unconjugated

Mouse IgG (Rat adsorbed) goat polyclonal antibody, Aff - Purified

Applications ELISA.
Flow Cytometry.
Reactivities Mouse
Conjugation Unconjugated