Human IgG (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/2,000. |
| Reactivities | Human |
| Conjugation | Unconjugated |







![Dot Blot showing the detection of Human IgG. A three-fold serial dilution of Human IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto (p/n [B501-0500]) 1 Hour at 20°C, Anti-Human IgG (H&L) (MOUSE) Antibody Fluorescien Conjugated (p/n 609-302-002) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/609-302-002-human-igg-antibody-fluorescein-conjugated-1-db-4x3.jpg?browse)

