Secondary Antibodies

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, Cy5

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation Cy5

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, BIMA

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation BIMA

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Cy5

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
Reactivities Rat
Conjugation Cy5

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, PE

Applications Flow Cytometry: < / = 0.02 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation PE

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone HIS40, Supernatant

Applications

Flow cytometry.
Immunofluorescence.
Immunohistochemistry on frozen tissue sections.

Reactivities Rat
Conjugation Unconjugated

Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA: 1:10,000/1:50,000
IF Microscopy: 1:1000/1:5000
Reactivities Rat
Conjugation FITC

Rat IgG (H+L chain), Fab Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/1000 - 1/2000.
Immunohistochemistry on frozen sections: 1/50.
Immunohistochemistry on paraffin sections: 1/50.
Immunohistochemistry on resin sections: 1/50.
Reactivities Rat
Conjugation HRP

Mouse IgA rabbit polyclonal antibody, Purified

Applications ELISA.
Reactivities Mouse, Rat
Conjugation Unconjugated

Rat IgG2a goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/500.
Reactivities Rat
Conjugation FITC

Rat IgG1 goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50 µl of 1/400 diluted antibody to label 1 x 106 cells in 100 µl.
Reactivities Rat
Conjugation FITC