Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified
| Applications | Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C. SDS-PAGE (12% separating gel). Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min. Strongly reacts with Bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry on Frozen Sections. |
| Reactivities | Bovine, Sheep |
| Conjugation | Unconjugated |




![Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody. Combination of anti-human IgE (BE5) FITC and anti-human CD63 (MEM-259) PE-Dyomics 647 ([SM3035P6]) was used (analysis in basophil window). <strong>Figure 1:</strong> staining of non-stimulated (control) sample.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/0101345.jpeg?browse)
