Secondary Antibodies

Mouse IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Human IgE goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Western Blot: 1/1000-1/5000.
Reactivities Human, Monkey
Conjugation AP

Rabbit Monoclonal Anti-Human IgG1 Antibody, Clone RM117

Applications ELISA: 50ng/well - 200ng/well for Capture;, 0.05ug/mL - 0.2ug/mL for Detection;, ICC: 0.5ug/mL-2ug/mL, IHC: 0.5ug/mL-2ug/mL
Reactivities Human IgG1
Conjugation Unconjugated

Rabbit IgG (H+L chain) goat polyclonal antibody, Biotin, Lyophilized

Applications Western Blot: 1/5,000-1/20,000 for Western blots.
ELISA: 1/50,000-1/200,000.
Immunohistochemistry: 1/1,000-1/5,000.

Reactivities Rabbit
Conjugation Biotin

Mouse lambda light chain rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/50,000 .
Western Blot: 1/1,000 - 1/5,000.
Immunohistochemistry on paraffin sections: 1/500 - 1/2,500.
Reactivities Mouse
Conjugation HRP

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, AP

Applications ELISA: 1/500-1/1,000.
Western blotting.
Dot- and Slot-Immunoblotting
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation AP

Canine IgE mouse monoclonal antibody, clone E6-2A1, Purified

Applications Western Blot.
ELISA: The antibody may be used as a Capture antibody in ELISA in combination with SM1499B as detecting antibody.
Reactivities Canine
Conjugation Unconjugated

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, TRITC

Applications Can be used to identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two immunoconjugates with different labels but originating from the same host animal species are used for direct two-color staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80.
Reactivities Rabbit
Conjugation TRITC

Monkey IgG (Fab specific) rabbit polyclonal antibody, TRITC

Applications Can be used for direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:10 and 1:40.
Reactivities Monkey
Conjugation TRITC

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), HRP

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation HRP

Rabbit Monoclonal Anti-Mouse IgM (mu-Chain) antibody, clone RM109

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL
Reactivities Mouse IgM mu-chain
Conjugation Unconjugated

Goat Monoclonal Anti-Rabbit IgG Fab antibody, clone RMG01

Applications ELISA: 0.01ug/mL – 0.5ug/mL; ICC/IHC: 0.5ug/mL-2ug/mL; WB (non-reduced): 0.1ug/mL-0.5ug/mL
Reactivities Rabbit IgG Fab
Conjugation Unconjugated

Porcine IgM mouse monoclonal antibody, clone 206-2, Aff - Purified

Applications ELISA: 0.1 µg/ml (1/4000).
Immunoprecipitation.
Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/2000).
Immunohistochemistry on Paraffin Sections (Does not react on routinely processed Paraffin Section).
Does not react in Western Blot with reduced and denatured samples.
Reactivities Porcine
Conjugation Unconjugated