Secondary Antibodies

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone HIS40, Supernatant

Applications

Flow cytometry.
Immunofluorescence.
Immunohistochemistry on frozen tissue sections.

Reactivities Rat
Conjugation Unconjugated

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on paraffin sections.
Reactivities Mouse
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone MH25/1, Purified

Applications Immunofluorescence.
Immunohistochemistry on frozen and paraffin sections: 1/5 – 1/10 (Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but the optimal dilution should be tested by serial dilution.)
Dilute immediately before use with PBS or TRIS.
Localisation: D ε 1 epitope on Fc Fragment
Positive control: Polypus nose
Reactivities Human
Conjugation Unconjugated

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-2, Aff - Purified

Applications Immunoassay.
Immunohistochemistry on Frozen Sections.
The specificity of monoclonal antibody anti-IgG subclasses is determined in a range of optimal concentrations. Increasing of the first or second antibody at a high level can induce cross-reactions with other IgG subclasses.
Reactivities Mouse
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone B572M, Purified

Applications ELISA.
Lateral flow assays.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone MK1A6, Purified

Applications ELISA assay development of total IgG.
Passive hemagglutination assays.
Histological staining of Acetone Fixed Frozen tissue sections.
Reactivities Human
Conjugation Unconjugated

Feline IgG mouse monoclonal antibody, clone GPB2-2B1, Purified

Applications ELISA: This antibody can bind to virus-bound immune IgG and can therefore detect anti-FeLV, FIV, FCV, FHV, FCoV/FIPV and FPV IgG activity in ELISA.
Western Blot: This antibody detects a band of approximately 50.0kDa on purified Feline IgG under reducing conditions.
Reactivities Feline
Conjugation Unconjugated

Human IgG (Fc CH2 Domain specific) mouse monoclonal antibody, clone 8A4, Purified

Applications ELISA.
Flow cytometry (1:50/1:100): use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Human
Conjugation Unconjugated

Monkey IgA mouse monoclonal antibody, clone IgA5-3B, Purified

Applications ELISA.
Western Blot: Detects a band of approximately 55kDa under reducing conditions.
Reactivities Feline, Monkey
Conjugation Unconjugated

Mouse IgM (Mu chain specific), F(ab)2 Fragment rat monoclonal antibody, clone SB73a, Purified

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgM+.
Immunofluorescence Microscopy: Identification and enumeration of IgM+.
ELISA: < / = 1 µg/ml.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, Low Endotoxin

Applications Flow Cytometry (See Ref.1-4).
Functional Assays.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Low Endotoxin

Applications Functional Assays.
Reactivities Mouse
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 090-11348, Purified

Applications ELISA.
Recommended antibody pair for Sandwich Immunoassay:
Capture / Detection
AM00919PU-N / AM00918PU-N
Reactivities Human
Conjugation Unconjugated

Human IgA (secretory) mouse monoclonal antibody, clone B781M, Purified

Applications ELISA: 1/20,000 dilution will yield > 1.0 O.D. using Alkaline Phosphatase conjugated Rabbit anti-Mouse Ig.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 090-11368, Purified

Applications ELISA.
Recommended pair for Sandwich Immunoassay
Capture: AM00919PU-N
Detection: AM00917PU-N
Reactivities Human
Conjugation Unconjugated