Human IgG2 (Hinge Region) mouse monoclonal antibody, clone 3C7, Purified
| Applications | ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Secondary Antibodies
Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified
| Applications | Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C. SDS-PAGE (12% separating gel). Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min. Strongly reacts with Bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry on Frozen Sections. |
| Reactivities | Bovine, Sheep |
| Conjugation | Unconjugated |
CNY 7710.00
5周
Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Purified
| Applications | To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgE mouse monoclonal antibody, clone 904, Purified
| Applications | ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
IgG (Immunglobulin G, light chain), mouse anti porcine, clone PHE425
| Applications | Tested for immunohistochemistry (IHC), ELISA and Western Blot, other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.1µg/ml (1:2000) IHC, paraffin sections: 0.25µg/ml (1:800); Proteinase K pretreatment for antigen retrieval is recommended. Western Blot: 0.05 – 0.5µg/ml. ELISA: 20 - 50ng/ml (EC50 of 1ng/ml illustrated below) Optimal dilutions should be determined by the end user. |
| Reactivities | Porcine |
| Conjugation | Unconjugated |
CNY 3113.00
4周
Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, Purified
| Applications | Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/106 cells. ELISA. Immunohistochemistry on Frozen Sections. Western blotting. Dot- and Slot-Immunoblotting. Immunocytochemistry. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 7710.00
5周
Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), Purified
| Applications | To identify the presence of IgG3 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG3, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Rabbit Monoclonal Anti-Human Gamma Heavy Chain antibody, clone RMG02
| Applications | ELISA: 0.05ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL |
| Reactivities | Human gamma heavy chain |
| Conjugation | Unconjugated |
Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified
| Applications | Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. Preparation and Pretreatment: 1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C. 2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step. Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgA (secretory) mouse monoclonal antibody, clone SC-05, Purified
| Applications | Western blot. Immunoprecipitation. Immunohistochemistry on Paraffin Sections. Immunohistochemistry on Frozen Sections. Examples of positive Human tissues: Secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 6190.00
5周
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Ascites
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: from 1/50-1/200. ELISA: form 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgE mouse monoclonal antibody, clone 090-18132, Purified
| Applications | ELISA. Recommended pairs for Sandwich Immunoassay: Capture / Detection: AM00919PU-N / AM00917PU-N. AM00919PU-N / AM00918PU-N. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, Purified
| Applications | ELISA: 1/4,000-1/8,000. Flow Cytometry. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
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Reactivities
- Human (114)
- Mouse (42)
- Rat (21)
- Chicken (9)
- Rabbit (5)
- Duck (3)
- Feline (3)
- Canine (2)
- Porcine (2)
- Bovine (1)
- Equine (1)
- Human IgA (1)
- Human IgA1 (1)
- Human IgA2 (1)
- Human IgD (1)
- Human IgE (1)
- Human IgG1 (1)
- Human IgG3 (1)
- Human IgM (1)
- Human gamma heavy chain (1)
- Human kappa light chain (1)
- Human lambda light chain (1)
- Monkey (1)
- Mouse Ig kappa light chain (1)
- Mouse IgG gamma (1)
- Mouse IgG1 (1)
- Mouse IgG2a kappa (1)
- Mouse IgG2b (1)
- Mouse IgM lambda light chain (1)
- Mouse IgM mu-chain (1)
- Rabbit IgG Fab (1)
- Rabbit IgG Fc (1)
- Sheep (1)
