Human IgE (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to identify and measure IgE in Human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80, depending on the method used. |
| Reactivities | Human |
| Conjugation | FITC |




![Dot Blot showing the detection of Human IgG. A three-fold serial dilution of Human IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto (p/n [B501-0500]) 1 Hour at 20°C, Anti-Human IgG (H&L) (MOUSE) Antibody Fluorescien Conjugated (p/n 609-302-002) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/609-302-002-human-igg-antibody-fluorescein-conjugated-1-db-4x3.jpg?browse)
