Secondary Antibodies

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Mouse IgM (Fc specific) Rabbit Polyclonal Antibody
AP32992TC-N

CNY 7710.00

5周

Mouse IgM (Fc specific) rabbit polyclonal antibody, TRITC

Applications ELISA.
Immunocytochemistry.
(In)direct immunofluorescence
Immunohistochemistry on Frozen Sections.
Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/10- 1/80.
Reactivities Mouse
Conjugation TRITC
Mouse IgM (Fc specific) Rabbit Polyclonal Antibody
AP32992FC-N

CNY 7710.00

5周

Mouse IgM (Fc specific) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
(In)direct immunofluorescence.
In direct staining of cytoplasmic IgM in fixed mouse cells and tissue substrates; to identify circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20- 1/80.
Reactivities Mouse
Conjugation FITC
Mouse IgM (Fc specific) Rabbit Polyclonal Antibody
AP32992AF-N

CNY 6580.00

5周

Mouse IgM (Fc specific) rabbit polyclonal antibody, Azide Free

Applications Can be used:
As unlabelled primary or secondary antibody reagent for indirect detection of IgM in Mouse cells, tissues and body fluids in Immunofluorescence and Immunoenzyme assay methods.
For the production of immunoconjugates with a selected marker.
To prepare immunoaffinity adsorbents by coupling to an artificial carrier.
In non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent.
In Western blotting.
When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling techniques, the optimum concentration of this product should be established before.
Recommneded Working Dilutions:
Histochemistry: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Precipitin titre: not less than 1/64 when tested against normal Mouse serum in agar-block immunodiffusion titration.

Performance testing: Titre, specificity and reactivity of the subclass specific cross-adsorbed IgG is further evaluated in a number of highly sensitive non-precipitating antibody-binding assay systems. These performance tests include direct single and double identification of cIg in mouse cell and tissue substrates and their evaluation as second antibody in the analysis of human cells and tissues after reacting with a primary monoclonal mouse antibody to a human antigen. The quantitative specific recognition ability is verified in double staining procedures together with reference reagents of known specificity and reactivity.
Reactivities Mouse
Conjugation Unconjugated
Mouse IgG (Fc specific) Rabbit Polyclonal Antibody
R1352P

CNY 2657.00

4周

Mouse IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications Immunoprecipitation.
Immunodiffusion.
Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1/40,000-1/180,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated
Mouse IgG (H+L chain), adsorbed Donkey Polyclonal Antibody
R1348HRP

CNY 4538.00

4周

Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/2,000-1/20,000 for Western blot), ELISA (1/180,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/1,000-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/80,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation HRP
Mouse lambda light chain Goat Polyclonal Antibody
SP2185P

CNY 7425.00

4周

Mouse lambda light chain goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/4000 - 1/8000.
Western Blot.
Immunofluorescence.
Immunoblotting.
Reactivities Mouse
Conjugation Unconjugated
Mouse IgG (H+L chain), F(ab)2 Fragment Rabbit Polyclonal Antibody
SP1012B

CNY 11960.00

4周

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Biotin

Applications ELISA: 1/1000 - 1/2000.
Flow Cytometry: 1/50 - 1/100; Use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunohistochemistry on frozen and paraffin embedded sections: 1/50 - 1/200.
Reactivities Mouse
Conjugation Biotin
Mouse IgG (H&L) Antibody Pre-adsorbed
TA397814

CNY 2963.00

4周

Mouse IgG (H&L) Antibody Pre-adsorbed

Applications WB: 1:2,000 - 1:20,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:50,000 - 1:100,000
Reactivities Mouse
Conjugation Unconjugated
Fluorescein Conjugated Mouse IgG (H&L)
TA397848

CNY 1181.00

4周

Fluorescein Conjugated Mouse IgG (H&L)

Applications WB: User Optimized
IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation FITC
Mouse IgM (mu chain) Antibody
TA397845

CNY 3285.00

4周

Mouse IgM (mu chain) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,400 - 1:40,400
Reactivities Mouse
Conjugation Unconjugated
Mouse IgG3 Antibody Pre-adsorbed
TA397813

CNY 3188.00

4周

Mouse IgG3 Antibody Pre-adsorbed

Applications WB: 1:4,000 - 1:20,000
IHC: 1:2,000 - 1:10,000
ELISA: 1:40,000 - 1:200,000
Reactivities Mouse
Conjugation Unconjugated
Mouse IgG (H&L) Secondary Antibody Biotin Conjugated
TA397920

CNY 1744.00

4周

Mouse IgG (H&L) Secondary Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:200,000 - 1:300,000
Reactivities Mouse
Conjugation Biotin
Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed
TA397856

CNY 1950.00

4周

Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: User Optimized
ELISA: 1:100,000
Reactivities Mouse
Conjugation HRP
Mouse IgG2a Antibody ATTO 488 Conjugated Pre-adsorbed
TA397867

CNY 5888.00

4周

Mouse IgG2a Antibody ATTO 488 Conjugated Pre-adsorbed

Applications WB: 1:4,000 - 1:20,000
IF: >1:5,000
FC: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 488
Mouse IgG3 Antibody ATTO 425 Conjugated Pre-adsorbed
TA397863

CNY 5888.00

4周

Mouse IgG3 Antibody ATTO 425 Conjugated Pre-adsorbed

Applications WB: 1:4,000 - 1:20,000
IF: >1:5,000
FC: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 425