Human IgA alpha Antibody Peroxidase Conjugated
| Applications | WB: 1:1,000 - 1:5,000 IHC: 1:500 - 1:2,500 ELISA: 1:10,000 - 1:200,000 |
| Reactivities | Human |
| Conjugation | HRP |
Secondary Antibodies
Human IgG (H+L chain), adsorbed goat polyclonal antibody, HRP
| Applications | ELISA: 1/200,000.
Western Blot: 1/1,000-1:5,000.
Immunohistochemistry: 1/500-1/2,500 |
| Reactivities | Human |
| Conjugation | HRP |
Human IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/300,000 Western Blot: 1/5,000-1/25,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgG F(ab')2 Antibody Peroxidase Conjugated
| Applications | WB: 1:1,000 - 1:10,000 IHC: 1:500 - 1:2,500 ELISA: 1:10,000 - 1:50,000 |
| Reactivities | Human |
| Conjugation | HRP |
Human IgG (H&L) Antibody
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:20,000 - 1:100,000 |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgA + IgG + IgM goat polyclonal antibody, FITC
| Applications | Flow Cytometry: Use 10 µl of 0.1-0.5 µg/ml diluted antibody to label 106 cells in 100 µl. |
| Reactivities | Human |
| Conjugation | FITC |
Human IgM rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes. Positive control: Tonsil. Cellular localization: Cytoplasmic. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes. Positive control: Tonsil. Cellular localization: Cytoplasmic. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (H&L) Antibody Rhodamine Conjugated
| Applications | IF: 1:500-1:2,500 |
| Reactivities | Human |
| Conjugation | TRITC |
Human IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/10,000. Immunohistochemistry: 1/500-2,500. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgG (gamma chain specific) rabbit polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:35,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Human |
| Conjugation | Biotin |
Human IgE (Fc specific) goat polyclonal antibody, Azide Free
| Applications | This antiserum is intended to detect and identify IgE in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Antibody titre: Precipitin titre not less than 1/16 when tested against an appropriate reference in agar immunodiffusion block titration. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgD (Fc specific) goat polyclonal antibody, Azide Free
| Applications | This antiserum is intended to detect and identify IgD in serum or other body fluids using the Immunofixation technique. Like Immunoelectrophoresis Immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. Antibody titre: Precipitin titre not less than 1:16 when tested against an appropriate reference in agar immunodiffusion block titration. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (H+L chain) chicken polyclonal antibody, FITC
| Applications | Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues. |
| Reactivities | Human |
| Conjugation | FITC |
Human IgE goat polyclonal antibody, Biotin
| Applications | ELISA: 1/10,000-1/100,000. Western Blot: 1/10,000-1/100,000. |
| Reactivities | Human, Monkey |
| Conjugation | Biotin |
