Mouse IgG2b Antibody ATTO 532 Conjugated
| Applications | WB: 1:4,000 - 1:20,000 IF: >1:5,000 FC: 1:500 - 1:2,500 FLISA: >1:20,000 |
| Reactivities | Mouse |
| Conjugation | ATTO 532 |
Secondary Antibodies
Mouse IgG2a Antibody Rhodamine Conjugated Pre-adsorbed
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 IF: 1:1,000 - 1:5,000 FLISA: 1:20,000 - 1:100,000 |
| Reactivities | Mouse |
| Conjugation | TRITC |
Mouse IgG2b Antibody ATTO 425 Conjugated Pre-adsorbed
| Applications | WB: 1:4,000 - 1:20,000 IF: >1:5,000 FC: 1:500 - 1:2,500 FLISA: >1:20,000 |
| Reactivities | Mouse |
| Conjugation | ATTO 425 |
Mouse IgM (H&L) Antibody Peroxidase Conjugated
| Applications | WB: 1:1,000 - 1:10,000 IHC: 1:500 - 1:2,500 ELISA: 1:10,000 - 1:50,000 |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgG (H+L chain) rabbit polyclonal antibody, Ig Fraction
| Applications | Immunoprecipitation. Immunodiffusion. Ouchterlony Test: Sample size: 15 µl. Distance between wells: 5 mm. Test Time: 5 days. Temperature: 37°C. Samples Tested: Normal mouse Serum (NMS), Purified mouse IgG, Normal human serum (NHS), Purified human IgG. Results: Specificity: a single band against NMS was detected at dilutions of 1/4 and 1/8 which is homogeneous with band to purified mouse IgG. No bands were detected against human IgG at 5 mg/ml or NHS at 1/4 dilution. Protein content: Biuret Assay 14 mg/ml. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, HRP
| Applications | ELISA: 1/500-1/1000. Immunohistochemistry on Frozen Sections: 1/50. Immunohistochemistry on Paraffin Sections. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgG (H+L chain) goat polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/150,000. Western Blot: 1/3,000-1/15,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgM (Fc specific) goat polyclonal antibody, TRITC
| Applications | Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/10 and 1/80. |
| Reactivities | Mouse |
| Conjugation | TRITC |
Mouse IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: For histochemical and Cytochemical use are usually between 1/100 and 1/250 In ELISA and comparable non-precipitating antibody-binding assays between 1/1500 and 1/6000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgM (Fc specific) goat polyclonal antibody, FITC
| Applications | In direct staining of cytoplasmic IgM in fixed mouse cells and tissue substrates; to identify circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/80. |
| Reactivities | Mouse |
| Conjugation | FITC |
Mouse IgA (alpha chain specific) goat polyclonal antibody, PE
| Applications | FLISA. Flow Cytometry. Immunofluorescence: ≤ 0.1 µg/106 cells. Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | PE |
Mouse IgA (alpha chain specific) goat polyclonal antibody, Aff - Purified
| Applications | Immunoblotting. Flow Cytometry. Immunohistochemistry. Enzyme-Linked-Immunosorbent-Assay (ELISA). Fluorescent-Linked-Immunosorbent-Assay (FLISA). |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Aff - Purified
| Applications | Indirect Immunostaining (In conjunction with mouse monoclonal antibodies). |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG3 Antibody Fluorescein Conjugated Pre-adsorbed
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 IF: 1:1,000 - 1:5,000 FC: 1:200 - 1:500 FLISA: 1:10,000 - 1:50,000 |
| Reactivities | Mouse |
| Conjugation | FITC |
Mouse IgG2b Antibody Fluorescein Conjugated Pre-adsorbed
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 IF: 1:1,000 - 1:5,000 FC: 1:200 - 1:500 FLISA: 1:10,000 - 1:50,000 |
| Reactivities | Mouse |
| Conjugation | FITC |
